caspase-7 p20 Antibody (B-5)recommended for detection of p20 subunit and precursor of caspase-7 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

caspase-7 p20 Antibody (B-5): sc-28295

caspase-7 p20 Antibody (B-5) is rated 5.0 out of 5 by 7.
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Datasheets

    • caspase-7 p20 Antibody (B-5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 15-50 near the N-terminus of caspase-7 p20 of human origin
    • caspase-7 p20 Antibody (B-5) is recommended for detection of p20 subunit and precursor of caspase-7 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine
    • caspase-7 p20 Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-28295 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-7 p20 (B-5): sc-28295.
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What application is the blocking peptide sc-28295 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Western blot This antibody work well in western blot (gastric cancer cell line: AGS, MKN45, dilute 1:1000)
Date published: 2017-11-14
Rated 5 out of 5 by from high sensitivity The first thing to strike us was the amazing high specificity, sensitivity, convenience and quickness of the caspase-7 p20 antibody . It is really excellent.
Date published: 2017-10-18
Rated 5 out of 5 by from target signal I used this antibody for WB analysis,I analysis the mouse colon tissue expression of the target protein,I got the target band and no addtional bands seen.
Date published: 2017-07-29
Rated 5 out of 5 by from import The cysteine protease family is a specific target protein that is critical for the destruction of cell life. And we usually know that polymerase in DNA mutation and double-strand breaks in the measurement plays an indispensable role. This is our test in the oxygen-sliding process important test items
Date published: 2017-02-06
Rated 5 out of 5 by from Amazing IHC(P) staining caspase-7 p20 (B-5) gave beautiful IHC(P) staining in human colon tissue showing cytoplasmic and nuclear staining of glandular cells. Also works really well for Western blot (WB).
Date published: 2017-02-03
Rated 5 out of 5 by from Good specificity for IHC(P) staining Good specificity for IHC(P) staining. -SCBT QC
Date published: 2015-02-04
Rated 5 out of 5 by from Great detection of the p20 cleavage product Great detection of the p20 cleavage product when HeLa or Jurkat are treated with UV or Staurosporine. -SCBT QC
Date published: 2014-11-12
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