caspase-7 Antibody (51)recommended for detection of p20 subunit and full length caspase-7 of human and canine origin by WB and IF

caspase-7 Antibody (51): sc-135858

caspase-7 Antibody (51) is rated 4.0 out of 5 by 2.
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Datasheets

    • caspase-7 Antibody (51) is a mouse monoclonal IgG2b provided at 50 µg/500 µl
    • raised against amino acids 4-126 of caspase-7 of human origin
    • recommended for detection of p20 subunit and full length caspase-7 of human and canine origin by WB and IF; not recommended for IP
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What is the recommended fixation method for immunofluorescence staining with caspase-7 (51): sc-135858 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Blowout band at expected molecular weight Blowout band at expected molecular weight with Hep G2 whole cell lysate with one other non-specific band. -SCBT QC
Date published: 2015-03-05
Rated 4 out of 5 by from Bands at expected size with transfected cell Bands at expected size with transfected cell line with minimal background -SCBT QC
Date published: 2014-01-27
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