caspase-6 p10 Antibody (H-12)recommended for detection of p10 subunit and precursor of caspase-6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

caspase-6 p10 Antibody (H-12): sc-377393

caspase-6 p10 Antibody (H-12) is rated 4.8 out of 5 by 4.
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Datasheets

    • caspase-6 p10 Antibody (H-12) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 194-253 of caspase-6 p10 of human origin
    • caspase-6 p10 Antibody (H-12) is recommended for detection of p10 subunit and precursor of caspase-6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • caspase-6 p10 Antibody (H-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-6 p10 (H-12): sc-377393.
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Can caspase-6 p10 (H-12): sc-377393 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, caspase-6 p10 (H-12): sc-377393 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Excellent for WB it works excellent for WB in our lab, will keep usinig it
Date published: 2017-09-18
Rated 4 out of 5 by from Very good antibody for Western blotting This product has been independently tested and validated in-house at Kinexus Bioinformatics Corporation as a very potent and highly specific antibody for Western blotting with a mixture of lysates from human cancer cell lines (A431/HepG2/MCF7). It strongly detects appropriately sized ~33 and ~10 kDa target proteins in immunoblots. However, it generates a higher than normal background.
Date published: 2016-10-13
Rated 5 out of 5 by from Robust staining in paraffin Robust staining in paraffin-embedded human small intestine. -SCBT QC
Date published: 2015-03-18
Rated 5 out of 5 by from Clean blot with single strong band at expected Clean blot with single strong band at expected size in Jurkat cell lysates. -SCBT QC
Date published: 2014-05-16
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