caspase-6 p10 Antibody (H-12) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
raised against amino acids 194-253 of caspase-6 p10 of human origin
Anti-caspase-6 p10 Antibody (H-12) is recommended for detection of p10 subunit and precursor of caspase-6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Anti-caspase-6 p10 Antibody (H-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-6 p10 (H-12): sc-377393.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
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Please note: We can not ship to PO boxes
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
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Labware ships FedEx Ground free of charge to the contiguous US
A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, Ced-3/caspase-1, is comprised of caspase-1, caspase-2, caspase-3, caspase-4, caspase-6, caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9 and caspase-10. Ced-3/caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Poly(ADP-ribose) polymerase plays an integral role in surveying for DNA mutations and double strand breaks. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the nuclear protein PARP into an apoptotic fragment. Caspase-6, but not caspase-3, has been shown to cleave the nuclear lamins which are critical to maintaining the integrity of the nuclear envelope and cellular morphology. Caspase-10 has been shown to activate caspase-3 and caspase-7 in response to apoptotic stimuli. Human caspase-6 is expressed as two isoforms, one of which is designated caspase-6 p10 and may be phosphorylated on Ser 257.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Can caspase-6 p10 (H-12): sc-377393 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?
Asked by: jerojero
Thank you for your question. Yes, caspase-6 p10 (H-12): sc-377393 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here:
Rated 5 out of
Excellent for WBit works excellent for WB in our lab, will keep usinig it
Date published: 2017-09-18
Rated 4 out of
Steven Pelech at Kinexus from
Very good antibody for Western blottingThis product has been independently tested and validated in-house at Kinexus Bioinformatics Corporation as a very potent and highly specific antibody for Western blotting with a mixture of lysates from human cancer cell lines (A431/HepG2/MCF7). It strongly detects appropriately sized ~33 and ~10 kDa target proteins in immunoblots. However, it generates a higher than normal background.
Date published: 2016-10-13
Rated 5 out of
Robust staining in paraffinRobust staining in paraffin-embedded human small intestine. -SCBT QC
Date published: 2015-03-18
Rated 5 out of
Clean blot with single strong band at expectedClean blot with single strong band at expected size in Jurkat cell lysates. -SCBT QC
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