caspase-4 Antibody (4B9)recommended for detection of caspase-4 of human origin by WB and IP

caspase-4 Antibody (4B9): sc-56056

caspase-4 Antibody (4B9) is rated 5.0 out of 5 by 7.
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Datasheets

    • caspase-4 Antibody (4B9) is a mouse monoclonal IgG1 provided at 100 µg/ml
    • raised against amino acids 1-270 of caspase-4 of human origin
    • recommended for detection of caspase-4 of human origin by WB and IP; may cross-react with Myc-tagged caspase-4 protein expressed in 293T cells
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-4 (4B9): sc-56056.
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May I ask can this antibody be used to detect the cleaved form of caspase-4?? #caspase-4 Antibody (4B9): sc-56056

Asked by: Angelawang
Thank you for your question. This mouse monoclonal antibody, caspase-4 (4B9): sc-56056, has not been used before for detection of the specific apoptotic cleaved forms of caspase-4.
Answered by: Technical Support
Date published: 2017-10-13

Does this antibody have an associated blocking peptide?

Asked by: AbPolly
Thank you for your question. As this antibody was raised against a recombinant protein and not a short peptide sequence, it is not possible to offer a blocking peptide for this antibody.
Answered by: Technical Service 11
Date published: 2017-01-12

Can this antibody detect mouse caspase-4?

Asked by: AbPolly
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Service 11
Date published: 2017-01-12
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Rated 5 out of 5 by from Worked really well for me I got this antibody two weeks and I run a western blot on THP-1 cell lysate using 30ug of protein the result was stunning!! I use it in combination with sc-516102 and I got very clear bands and non-specific binding were NOT detected in any membrane. What can I say great product (as always) and competent technical service. The only issue I had was that I had to use a long exposure time.
Date published: 2018-03-13
Rated 5 out of 5 by from 抗体很不错。 非常感谢厂家给我送的试用装,我做的血管内皮细胞总蛋白,一抗1:500稀释,条带很清楚,但是由非特异性条带,除了90、50处有条带,30左右也有条带。
Date published: 2017-09-21
Rated 5 out of 5 by from Strong western !! Superb western blot results; strong signal with low background
Date published: 2017-01-17
Rated 5 out of 5 by from This antibody will show you caspase-4! A great detection reagent of caspase-4. Reliable in WB procedures and certain to detect both monomers and dimers of the protein. It has been cited 10 times.
Date published: 2016-12-23
Rated 5 out of 5 by from WB positive with A549 and A2780 cells WB positive with A549 and A2780 cells. -SCBT Publication Review
Date published: 2015-03-30
Rated 5 out of 5 by from Great antibody for Western Great antibody for Western, strong caspase-4 band detected in hela whole cell lysates. -SCBT QC
Date published: 2015-03-04
Rated 5 out of 5 by from Great antibody for Western Great antibody for Western, strong dimer and monomer bands detected. -SCBT QC
Date published: 2015-01-14
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