Anti-caspase-3 p17 Antibody (G-5) recommended for detection of p17 subunit and full length precursor of caspase-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-caspase-3 p17 Antibody (G-5): sc-166589

Anti-caspase-3 p17 Antibody (G-5) is rated 5.0 out of 5 by 3.
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Datasheets

    • caspase-3 p17 Antibody (G-5) is a mouse monoclonal IgG2b κ, cited in 6 publications, provided at 200 µg/ml
    • raised against amino acids 56-104 mapping near the N-terminus of caspase-3 of human origin
    • recommended for detection of p17 subunit and full length precursor of caspase-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) is the preferred secondary detection reagent for caspase-3 p17 Antibody (G-5) for WB and IHC(P) applications. This reagent is now offered in a bundle with caspase-3 p17 Antibody (G-5) (see ordering information below).

Can caspase-3 p17 (G-5): sc-166589 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Trav11
Thank you for your question. Yes, caspase-3 p17 (G-5): sc-166589 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Works well for WB in human samples Works well for WB in human samples, publishable data. -SCBT Publication Review
Date published: 2015-02-23
Rated 5 out of 5 by from Good quality antibody for human paraffin samples Good quality antibody for human paraffin samples. -SCBT QC
Date published: 2015-01-12
Rated 5 out of 5 by from Clean blot with single strong band in CCRF Clean blot with single strong band in CCRF-CEM and Jurkat whole cell lysates. -SCBT QC
Date published: 2014-05-22
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