Anti-caspase-3 p17 Antibody (D-12) recommended for detection of caspase-3 p17 and full length procaspase-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-caspase-3 p17 Antibody (D-12): sc-373730

Anti-caspase-3 p17 Antibody (D-12) is rated 4.3 out of 5 by 30.
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Datasheets

    • caspase-3 p17 Antibody (D-12) is a mouse monoclonal IgG2b κ, cited in 35 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 50-86 within an internal region of caspase-3 p17 of human origin
    • Anti-caspase-3 p17 Antibody (D-12) is recommended for detection of caspase-3 p17 and full length procaspase-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-caspase-3 p17 Antibody (D-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-3 p17 (D-12): sc-373730.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred secondary detection reagent for caspase-3 p17 Antibody (D-12) for WB and IHC(P) applications. This reagent is now offered in a bundle with caspase-3 p17 Antibody (D-12) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

What application is the blocking peptide sc-373730 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Quite good antibody 1:200 diluted for wb of rat protein ,the band is at 15kd
Date published: 2019-06-17
Rated 3 out of 5 by from It was great It was great but doesn't work well after a new batch.
Date published: 2018-06-29
Rated 5 out of 5 by from caspase-3 p17 Western blot of mouse primary cerebellar neuron conjugated with caspase-3 p17 (1:1000). Protein concentration 30 µg per lane. Strong expression with low background.
Date published: 2018-03-09
Rated 5 out of 5 by from Good production we used TG mice Hip as sample,and the result as we expected,
Date published: 2018-03-01
Rated 4 out of 5 by from Weak signal at 17kDa on Western Blot. Detects doublet around 36kDa (uncleaved form) a weakly detects 17kDa band. Needs further optimizing.
Date published: 2018-01-31
Rated 4 out of 5 by from good antibody I have used the caspase-3 p17 antibody, the total band is so clear and the p17 band is less than the total band.
Date published: 2017-10-06
Rated 3 out of 5 by from weak bands can be seen you can see weak bands with the antibody. The Ph value of 1*TBS buffer is 7.45. I haven't try a buffer with lower Ph value.
Date published: 2017-09-30
Rated 5 out of 5 by from Great for early apoptosis detection Got this Ab as a sample, and tested on HEK cells, seems to work great.
Date published: 2017-09-25
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