Anti-caspase-3 p17 Antibody (B-4) recommended for detection of p17 subunit and full length precursor of caspase-3 of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-caspase-3 p17 Antibody (B-4): sc-271028

Anti-caspase-3 p17 Antibody (B-4) is rated 4.9 out of 5 by 11.
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Datasheets

    • caspase-3 p17 Antibody (B-4) is a mouse monoclonal IgG2a κ, cited in 53 publications, provided at 200 µg/ml
    • raised against amino acids 56-104 mapping near the N-terminus of caspase-3 p17 of human origin
    • caspase-3 p17 Antibody (B-4) is recommended for detection of p17 subunit and full length precursor of caspase-3 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-caspase-3 p17 Antibody (B-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-3 p17 (B-4): sc-271028.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for caspase-3 p17 Antibody (B-4) for WB and IHC(P) applications. These reagents are now offered in bundles with caspase-3 p17 Antibody (B-4) (see ordering information below).

Can this antibody recognize Caspase 3 after being cleaved? The size is about 17kDa.

Asked by: lidi
Thank you for your question. This monoclonal antibody is recommended for detection of the cleaved p17 subunit and full length precursor of caspase-3 of human origin. There is Western blot data on our website showing detection of the cleaved caspase-3 p17 in Staurosporine treated HeLa whole cell lysates.
Answered by: Technical Support
Date published: 2019-01-25

Is there a recommended positive control for this Ab?

Asked by: Rachie
Thank you for your question. We recommend using the following positive controls with this caspase-3 p17 Antibody (B-4): sc-271028: Untreated (sc-2200) and Staurosporine (sc-3510) treated HeLa (sc-24698) or Jurkat (sc-24719) whole cell lysates for detection of procaspase-3 and cleaved caspase-3 p17 expression in Western blot. Untreated (sc-2239) and Kendomycin (sc-202196) treated U-937 whole cell lysates for detection of procaspase-3 and cleaved caspase-3 p17 expression in Western blot. CCRF-CEM (sc-2225), Jurkat (sc-2204), K-562 (sc-2203), and Hep G2 (sc-2227) whole cell lysates for detection of procaspase-3 expression in Western blot.
Answered by: Technical Support
Date published: 2017-05-16

Can this antibody also recognize the p12 subunit of caspase 3?

Asked by: AbPolly
Thank you for your question. Our caspase-3 p17 sc-271028 is specific to the precursor form of caspase-3 and the p17 subunit, and will not detect the p12 subunit.
Answered by: Technical Support 2
Date published: 2017-01-24
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Rated 5 out of 5 by from Very good in Western blotting Pro and cleaved forms of caspase-3 were detectable in CCCP-treated K562 cells by Western blotting.
Date published: 2019-12-24
Rated 5 out of 5 by from Works Perfectly on WB This antibody works perfectly on WB analysis, incubation Overnight at 1/2000 in non dry fat milk
Date published: 2018-06-02
Rated 5 out of 5 by from Perfect clean detection 33 kDa (pro-caspase) band This Ab lit up in the correct Mw range (33 kDa) very quickly and cleanly. Unfortunately, we were unable to see the p17 fragment. We're currently optimising our cell culture incubations to detect this. Otherwise, very clean WB from this Ab. Would recommend. Conditions: 40 µg protein, 1º Ab 1/200, overnight at RT, 2º Ab, 1/10,000 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) #sc-516102, 2 hrs RT. Imaging: ECL.
Date published: 2017-05-09
Rated 5 out of 5 by from works well Western blotting detection of human cancer cells, get the right band.
Date published: 2017-04-05
Rated 5 out of 5 by from Great for Immunoperoxidase This is antibody using Immunoperoxidase staining of human duodenum tissue, and it's very specific and clear.
Date published: 2017-03-28
Rated 5 out of 5 by from WB: clean Worked great on both our human samples for WB, 1:1000 dilution, very well established antibody.
Date published: 2017-02-12
Rated 5 out of 5 by from Works for detection of p17 subunit The (B-4) clone works for detection of the p17 subunit by WB in Staurosporine treated samples.
Date published: 2017-01-05
Rated 5 out of 5 by from Wonderful The memberane was blocked in 5% skim milk and probed with the caspase-3 antibody,the result is as:
Date published: 2016-11-24
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