caspase-3 p11 Antibody (C-6)recommended for detection of caspase-3 p11 subunit and full length procaspase-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

caspase-3 p11 Antibody (C-6): sc-271759

caspase-3 p11 Antibody (C-6) is rated 4.4 out of 5 by 25.
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Datasheets

    • caspase-3 p11 Antibody (C-6) is a mouse monoclonal IgG1 (lambda light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 185-211 at the N-terminus of caspase-3 p11 of human origin
    • caspase-3 p11 Antibody (C-6) is recommended for detection of caspase-3 p11 subunit and full length procaspase-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • caspase-3 p11 Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-271759 P
    • See m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-3 p11 (C-6): sc-271759.
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Hi, can you tell me if this mouse antibody works on mouse tissue for Immunohistochemistry. Thanks!

Asked by: IHC 1
Thank you for your question. Yes, this mouse monoclonal antibody, caspase-3 p11 (C-6): sc-271759, can be used for immunohistochemistry or IHC(P) on mouse tissue. A link to our recommended protocol is provided below as a reference. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-08-18

Is caspase-3 p11 (C-6), sc-271759, available directly conjugated to HRP or fluorescent dyes?

Asked by: TinTin
Thank you for your question! Yes, sc-271759 is available conjugated to agarose (sc-271759 AC), HRP (sc-271759 HRP), phycoerythrin (sc-271759 PE), fluorescein (sc-271759 FITC), Alexa Fluor® 488 (sc-271759 AF488) and Alexa Fluor® 647 (sc-271759 AF647).
Answered by: Technical Support 13
Date published: 2017-01-13

What is the best secondary to use with this antibody in Western Blot?

Asked by: TinTin
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-10
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Rated 5 out of 5 by from Western blot When tried 1:500 dilution,tried in human spleen tissue and the effect of this antibody was good, it always good band in 40kDa.
Date published: 2018-05-26
Rated 5 out of 5 by from Good production The product quality is very good, and the result of the band was very clear and strong signal.as we expected!
Date published: 2018-03-01
Rated 4 out of 5 by from good antibody Strong fast signal.. BUT with many unspecific band
Date published: 2017-12-21
Rated 4 out of 5 by from Works fine The antibody works fine, but shows some nonspecific bands in my sample.
Date published: 2017-11-17
Rated 4 out of 5 by from IHC Staining of Human Adenocarcinoma Cells I used this antibody to assess apoptosis in human adenocarcinoma cells using IHC. I was satisfied with it's performance and hope to use it in other applications in the future.
Date published: 2017-10-20
Rated 2 out of 5 by from weak bands you can see weak bands with this antibody. The sample is human cell line. Primary antibodies are diluted at 1:2000. The PH value of 1*TBS is 7.45.
Date published: 2017-09-30
Rated 5 out of 5 by from anticorps de grande qualité J'ai demandé des échantillons d'anticorps à tester. Les produits ont été livrés en moins de 24 heures. la communication est très claire et les informations sont obtenues immédiatement. des anticorps de grande qualité.
Date published: 2017-09-28
Rated 4 out of 5 by from Good antibody I got a clear band at 32kd, also nonspecific band at 58kd.
Date published: 2017-09-26
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