Anti-caspase-3 Antibody (7.1.44) recommended for detection of caspase-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-caspase-3 Antibody (7.1.44): sc-56051

Anti-caspase-3 Antibody (7.1.44) is rated 4.0 out of 5 by 1.
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Datasheets

    • caspase-3 Antibody (7.1.44) is a mouse monoclonal IgG2a κ, cited in 2 publications, provided at 200 µg/ml
    • raised against full length caspase-3 of human origin
    • recommended for detection of caspase-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See caspase-3 p11 (C-6): sc-271759 for caspase-3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred secondary detection reagent for caspase-3 Antibody (7.1.44) for WB and IHC(P) applications. This reagent is now offered in a bundle with caspase-3 Antibody (7.1.44) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

How much sample do you recommend loading on the gel for Western blot detection using caspase-3 (7.1.44): sc-56051?

Asked by: cjMara
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Good Western results with HT Good Western results with HT-29 and HCT-116 cells. -SCBT Publication Review
Date published: 2011-12-15
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