Anti-caspase-3 Antibody (4i29) recommended for detection of full-length caspase-3 at Mr 32 kDa and 17 kDa subunit of 10 ng recombinant of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-caspase-3 Antibody (4i29): sc-56050

Anti-caspase-3 Antibody (4i29) is rated 4.0 out of 5 by 1.
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Datasheets

    • caspase-3 Antibody (4i29) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against full length caspase-3 of human origin
    • recommended for detection of full-length caspase-3 at Mr 32 kDa and 17 kDa subunit of 10 ng recombinant of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See caspase-3 p11 (C-6): sc-271759 for caspase-3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for caspase-3 Antibody (4i29) for WB and IHC(P) applications. These reagents are now offered in bundles with caspase-3 Antibody (4i29) (see ordering information below).
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Can caspase-3 (4i29): sc-56050 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, caspase-3 (4i29): sc-56050 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 4 out of 5 by from Decent for WB in in CCRF Decent for WB in in CCRF-CEM (A) and MOLT-4 (B) whole cell lysates. -SCBT QC
Date published: 2015-02-04
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