caspase-3 Antibody (46) recommended for detection of p17 caspase-3 subunit and full length procaspase-3 of mouse, rat and human origin by WB, IP and IF

caspase-3 Antibody (46): sc-136219

caspase-3 Antibody (46) is rated 4.5 out of 5 by 2.
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Datasheets

    • caspase-3 Antibody (46) is a mouse monoclonal IgG1, cited in 40 publications, provided at 50 µg/0.5 ml
    • raised against amino acids 25-145 of caspase-3 of mouse origin
    • recommended for detection of p17 caspase-3 subunit and full length procaspase-3 of mouse, rat and human origin by WB, IP and IF
    • See caspase-3 p11 (C-6): sc-271759 for caspase-3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for caspase-3 Antibody (46). This work is in progress.
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caspase-3 (46): sc-136219 is IgG1 kappa light chain?

Asked by: pili1234
Thank you for your question. Unfortunately we do not have isotype information for this antibody at this time. Most likely it is kappa, but we can not say for sure.
Answered by: Tech Service
Date published: 2020-02-07

I am using caspase-3 (46): sc-136219 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jenniferc
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from This has worked very well in IP This has worked very well in IP
Date published: 2015-10-22
Rated 4 out of 5 by from Antibody generated positive WB data in transfected Antibody generated positive WB data in transfected 293T lysate and Jurkat whole cell lysate. -SCBT QC
Date published: 2014-04-04
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