caspase-3 Antibody (4.1.18)recommended for detection of caspase-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

caspase-3 Antibody (4.1.18): sc-65497

caspase-3 Antibody (4.1.18) is rated 5.0 out of 5 by 3.
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Datasheets

    • caspase-3 Antibody (4.1.18) is a mouse monoclonal mouse IgG2a κ (kappa light chain) provided at 200 µg/ml
    • raised against full length caspase-3 of human origin
    • recommended for detection of caspase-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See caspase-3 p11 (C-6): sc-271759 for caspase-3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Does it function to detect cleaved caspase 3?

Asked by: Dr Inês
Thank you for your question. We have used this mouse monoclonal antibody, caspase-3 (4.1.18): sc-65497, to successfully detect procaspase-3. We do not yet have data with this clone detecting cleaved caspase-3, but the optimal conditions for detection of cleaved caspase-3 may not yet be determined. This antibody has been cited in the literature at least 14 times. We would recommend consulting some of the product citations to see if anyone has used this clone for detection of the cleaved caspase-3.
Answered by: Technical Support
Date published: 2017-06-01

I am using caspase-3 (4.1.18): sc-65497 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Perfect I used this AB in WB and work perfectly, clear and nice bands.
Date published: 2019-05-13
Rated 5 out of 5 by from Well-rounded antibody for caspase-3 Works great for both WB and IHC on paraffin embedded tissue sections.
Date published: 2017-01-18
Rated 5 out of 5 by from Good for Western with human cell lysates Good for Western with human cell lysates -SCBT QC
Date published: 2014-12-04
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