caspase-3 Antibody (3F49)recommended for detection of caspase-3 of mouse, rat and human origin by WB, IP, IF and IHC(P)

caspase-3 Antibody (3F49): sc-70498

caspase-3 Antibody (3F49) is rated 4.5 out of 5 by 2.
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Datasheets

    • caspase-3 Antibody (3F49) is a mouse monoclonal mouse IgG2a κ (kappa light chain) provided at 200 µg/ml
    • raised against full length caspase-3 of human origin
    • recommended for detection of caspase-3 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See caspase-3 p11 (C-6): sc-271759 for caspase-3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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What is the recommended starting dilution for Western blot for caspase-3 (3F49): sc-70498 monoclonal antibody?

Asked by: jerojero
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-70498.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Single strong band at correct mw Single strong band at correct mw, Jurkat. -SCBT QC
Date published: 2015-02-13
Rated 4 out of 5 by from Performs well in Western with transfected lysate Performs well in Western with transfected lysate -SCBT QC
Date published: 2015-01-15
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