Anti-caspase-3 Antibody (3CSP01) recommended for detection of caspase-3 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-caspase-3 Antibody (3CSP01): sc-65496

Anti-caspase-3 Antibody (3CSP01) is rated 4.3 out of 5 by 3.
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Datasheets

    • caspase-3 Antibody (3CSP01) is a mouse monoclonal IgG2a κ, cited in 22 publications, provided at 200 µg/ml
    • raised against full length caspase-3 of human origin
    • recommended for detection of caspase-3 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See caspase-3 p11 (C-6): sc-271759 for caspase-3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for caspase-3 Antibody (3CSP01) for WB and IHC(P) applications. These reagents are now offered in bundles with caspase-3 Antibody (3CSP01) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-65496: caspase-3 (3CSP01) monoclonal antibody?

Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Excellent product The caspase-3 Antibody (3CSP01): sc-65496 works very well for Western blot applications, showing clear banding with minimal background.
Date published: 2017-02-03
Rated 4 out of 5 by from Antibody works with transfected lysate with Antibody works with transfected lysate with minimal background -SCBT QC
Date published: 2015-01-07
Rated 4 out of 5 by from Published with it in WB on human samples Published with it in WB on human samples. -SCBT Publication Review
Date published: 2014-02-20
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