caspase-3 Antibody (31A1067) is a mouse monoclonal IgG1, cited in 121 publications, provided at 100 µg/ml
raised against full length caspase-3 of human origin
recommended for detection of caspase-3 and full length procaspase-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
See caspase-3 p11 (C-6): sc-271759 for caspase-3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-3 (31A1067): sc-56053.
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for caspase-3 Antibody (31A1067) for WB and IHC(P) applications. These reagents are now offered in bundles with caspase-3 Antibody (31A1067) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
caspase-3 Antibody (31A1067): sc-56053 is IgG1 kappa light chain?
Asked by: pili1234
Thank you for your question. Unfortunately we do not have isotype information for this antibody at this time. Most likely it is kappa, but we can not say for sure.
Answered by: Tech Service
Date published: 2020-02-07
Est-ce que je peux avoir un devis pour l'achat de l'anticorps caspase-3 (31A1067): sc-56053;
Asked by: Hadj
Merci pour votre question. Veuillez contacter directement notre service clientèle et nous vous ferons parvenir immédiatement un devis pour ce produit.
'Please send me a quote for caspase-3 Antibody (31A1067): sc-56053.'
Thank you for your question. Please contact our customer service directly and we will send you a quotation for this product right away.
Answered by: Technical Support Europe
Date published: 2019-04-12
I need to use an antibody to detect the protein in samples of equine origin. Do you know if this mouse monoclonal antibody will work?
Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Rated 3 out of
Discreto funzionamentoHo comprato questo articolo 5 settimane fa. Provando condizioni diverse di wb sono riuscita ad ottenere un discreto segnale di bande
Date published: 2021-02-12
Rated 4 out of
Questions about the free samples(31A1067)The free samples(31A1067) can only detect procaspase-3. We can't detect the cleaved caspase-3 as you showed in the products specification. Do you offer specific antibody for it?
Date published: 2020-06-10
Rated 5 out of
Very good in Western blottingPro and cleaved forms of caspase-8 were detectable in CCCP-treated K562 cells by Western blotting at dilution of 1/100.
Date published: 2019-12-24
Rated 5 out of
Good antibody!1:200 diluted for wb of rat protein,the band is quite strong and special
Date published: 2019-06-17
Rated 5 out of
Foteini K from
Excellent antibody!!!I got a very strong band and without background in TBST 1x milk, dilution 1:250, in mouse tumor tissue!
Date published: 2018-05-11
Rated 2 out of
western blotting - inconsistent for cleaved bandUsed this antibody for western blotting of caspase-3 in whole AC16 cell lysates (40-50ug). Very faint bands seen at 17kD (cleaved protein) -background was dirty even after additional washes. Reproducibility was poor. The pro-caspase-3 band was robust, consistent and at the expected molecular weight.
Date published: 2018-04-27
Rated 5 out of
Great brandsI got the free samples last month, it works great. But just for the caspase-3, not the cleaved.
Date published: 2018-04-25
Rated 5 out of
Great product!!It's a great product for me to analyze Western blotting.
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn