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caspase-3 Antibody (31A1067) | SCBT - Santa Cruz Biotechnology
caspase-3 Antibody (31A1067) recommended for detection of caspase-3 and full length procaspase-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

caspase-3 Antibody (31A1067): sc-56053

caspase-3 Antibody (31A1067) is rated 4.5 out of 5 by 24.
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Datasheets

    • caspase-3 Antibody (31A1067) is a mouse monoclonal IgG1, cited in 223 publications, provided at 50 µg/0.5 ml
    • raised against full length caspase-3 of human origin
    • recommended for detection of caspase-3 and full length procaspase-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See caspase-3 p11 (C-6): sc-271759 for caspase-3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-3 (31A1067): sc-56053.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for caspase-3 Antibody (31A1067) for WB and IHC(P) applications. These reagents are now offered in bundles with caspase-3 Antibody (31A1067) (see ordering information below).
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I could not detect specific band on following condition on my western blot. ・Nitrocellulose membrane 0.45 µm ・C2C12 myoblast and C2C12 myoblast + H2O2 (500mM) ・Caspase-3 antibody: 1:200 ・IgGk antibody: 1:1000 Could you please give me some advice?

Asked by: SJWD
Thank you for your question.Please contact our Technical Service Department of Japan for more information.
Answered by: Jiajun Z
Date published: 2022-10-27

Can you recommend a compatible diluent buffer and concentration for this antibody? My blot showed up with a strong background and I was wondering what need to be modified?

Asked by: Saina
Thank you for your question. This antibody was established using UltraCruz® Blocking Reagent: sc-516214, which is a highly efficient plant protein based blocking buffer. You may also want to increase incubation time of the primary antibody to overnight incubation at +4° C, which allows for lowering the overall primary antibody concentration, and in consequence unspecific signals. If the issue persists, you may also want to consider using one of our secondary binding proteins, which circumvent unspecific signals from secondary detection in comparison to conventional polyclonal secondaries.
Answered by: Tech Support Europe
Date published: 2022-06-20

caspase-3 Antibody (31A1067): sc-56053 is IgG1 kappa light chain?

Asked by: pili1234
Thank you for your question. Unfortunately we do not have isotype information for this antibody at this time. Most likely it is kappa, but we can not say for sure.
Answered by: Tech Service
Date published: 2020-02-07

Est-ce que je peux avoir un devis pour l'achat de l'anticorps caspase-3 (31A1067): sc-56053; Merci

Asked by: Hadj
Merci pour votre question. Veuillez contacter directement notre service clientèle et nous vous ferons parvenir immédiatement un devis pour ce produit. 'Please send me a quote for caspase-3 Antibody (31A1067): sc-56053.' Thank you for your question. Please contact our customer service directly and we will send you a quotation for this product right away.
Answered by: Technical Support Europe
Date published: 2019-04-12

I need to use an antibody to detect the protein in samples of equine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 15
Date published: 2017-01-20
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Rated 5 out of 5 by from 质量不错 我两年前买了这管抗体,目前研究工作已经发表Bioactive Materials,标题为DNA aptamer S11e recognizes fibrosarcoma and acts as a tumor suppressor。值得推荐!
Date published: 2022-03-13
Rated 3 out of 5 by from Buena señal La señal es buena, pero algo sucia. La banda no sale bien definida
Date published: 2021-11-11
Rated 3 out of 5 by from Discreto funzionamento Ho comprato questo articolo 5 settimane fa. Provando condizioni diverse di wb sono riuscita ad ottenere un discreto segnale di bande
Date published: 2021-02-12
Rated 4 out of 5 by from Questions about the free samples(31A1067) The free samples(31A1067) can only detect procaspase-3. We can't detect the cleaved caspase-3 as you showed in the products specification. Do you offer specific antibody for it?
Date published: 2020-06-10
Rated 5 out of 5 by from Very good in Western blotting Pro and cleaved forms of caspase-8 were detectable in CCCP-treated K562 cells by Western blotting at dilution of 1/100.
Date published: 2019-12-24
Rated 5 out of 5 by from Good antibody! 1:200 diluted for wb of rat protein,the band is quite strong and special
Date published: 2019-06-17
Rated 5 out of 5 by from Excellent antibody!!! I got a very strong band and without background in TBST 1x milk, dilution 1:250, in mouse tumor tissue!
Date published: 2018-05-11
Rated 2 out of 5 by from western blotting - inconsistent for cleaved band Used this antibody for western blotting of caspase-3 in whole AC16 cell lysates (40-50ug). Very faint bands seen at 17kD (cleaved protein) -background was dirty even after additional washes. Reproducibility was poor. The pro-caspase-3 band was robust, consistent and at the expected molecular weight.
Date published: 2018-04-27
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