Thank you for your question. This antibody was established using UltraCruz® Blocking Reagent: sc-516214, which is a highly efficient plant protein based blocking buffer. You may also want to increase incubation time of the primary antibody to overnight incubation at +4° C, which allows for lowering the overall primary antibody concentration, and in consequence unspecific signals.
If the issue persists, you may also want to consider using one of our secondary binding proteins, which circumvent unspecific signals from secondary detection in comparison to conventional polyclonal secondaries.
Date published: 2022-06-20