caspase-2L Antibody (F-7)recommended for detection of p13 subunit, caspase-2L and full length caspase-2 precursor of mouse, rat and human origin by WB, IP, IF and ELISA

caspase-2L Antibody (F-7): sc-5292

caspase-2L Antibody (F-7) is rated 4.5 out of 5 by 2.
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Datasheets

    • caspase-2L Antibody (F-7) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 317-435 mapping at the C-terminus of caspase-2L of human origin
    • caspase-2L Antibody (F-7) is recommended for detection of p13 subunit, caspase-2L and full length caspase-2 precursor of mouse, rat and human origin by WB, IP, IF and ELISA; may cross-react with the p12 subunit
    • caspase-2L Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-2L (F-7): sc-5292.
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What blocking solution should I use with caspase-2L (F-7): sc-5292 monoclonal antibody for Western blot?

Asked by: AbPolly
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-12
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Rated 5 out of 5 by from Good results at 1:200 in Western with human Good results at 1:200 in Western with human U937 cells. - SCBT Publication Review
Date published: 2008-01-18
Rated 4 out of 5 by from Strong signal using 30 or 50 g of cell homogenate Strong signal using 30 or 50 g of cell homogenate in Western. - SCBT Publication Review
Date published: 2006-11-02
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