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caspase-2 Antibody (F-2) | SCBT - Santa Cruz Biotechnology
caspase-2 Antibody (F-2) recommended for detection of caspase-2S, caspase-2L, p18 subunit and full length caspase-2 precursor of mouse, rat and human origin by WB, IP, IF and ELISA

caspase-2 Antibody (F-2): sc-514472

caspase-2 Antibody (F-2) is rated 4.7 out of 5 by 3.
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Datasheets

    • caspase-2 Antibody (F-2) is a mouse monoclonal IgG1 κ caspase-2 antibody, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 153-297 of caspase-2S of human origin
    • caspase-2 Antibody (F-2) is recommended for detection of caspase-2S, caspase-2L, p18 subunit and full length caspase-2 precursor of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-caspase-2 Antibody (F-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-2 (F-2): sc-514472.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for caspase-2 Antibody (F-2) for WB applications. These reagents are now offered in bundles with caspase-2 Antibody (F-2) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-514472: caspase-2 (F-2) monoclonal antibody?

Asked by: Cweed
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 4 out of 5 by from Full length or 2L or 2S? The antibody works in WB and we see two bands in HEK293 and some tumor lines. My query is: what is the target of this antibody?— full length, 2L or 2S? In the datasheet, it is mentioned that the Ab was raised against 2S but the figure denotes full/2L. Clarification would be appreciated.
Date published: 2021-05-20
Rated 5 out of 5 by from Pretty good antibody!! Sc514472 works pretty well in identifying the active Caspase2 as well as the full length forms.
Date published: 2017-11-15
Rated 5 out of 5 by from Excellent for WB it works excellent for WB in our lab, will keep usinig it
Date published: 2017-09-18
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