caspase-11 p20 Antibody (A-2)recommended for detection of caspase-11 of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA

caspase-11 p20 Antibody (A-2): sc-374615

caspase-11 p20 Antibody (A-2) is rated 4.4 out of 5 by 9.
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Datasheets

    • caspase-11 p20 Antibody (A-2) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 141-195 of caspase-11 of mouse origin
    • caspase-11 p20 Antibody (A-2) is recommended for detection of caspase-11 of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA
    • caspase-11 p20 Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-11 p20 (A-2): sc-374615.
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What is the recommended secondary for this antibody?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-24

在43,35和25出现三个条带,请问这都是活性形式吗?哪个更有代表性

Asked by: panbingjie
感谢您的提问。caspase-11前体的分子量在40 kDa左右,p20亚基的分子量在20 kDa左右。欢迎您将您的实验结果发送到我们的技术支持部门,我们将就您的结果进行具体分析。我们的技术支持部门:电话(+86) 021-60936351,邮箱asia@scbio.cn。 Thank you for your question. Caspase-11 precursor has a molecular weight of 48 kDa, and the molecular weight of caspase-11 p20 subunit is 20 kDa. You are welcome to contact our Technical Services department at (+86) 021-60936351 or asia@scbio.cn to discuss your experimental outcome.
Answered by: Technical Support 12
Date published: 2016-12-01
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Rated 5 out of 5 by from clear band We got exactly clear band at the target position (rat fibroblast cell) ,strong signal for WB at 1:400.
Date published: 2018-01-27
Rated 1 out of 5 by from Did not work in WB of pancreatic lysates or HELA Received this as a sample. This antibody did not work in WB of mouse pancreatic lysates or HELA cells.
Date published: 2017-10-27
Rated 4 out of 5 by from 曝光条带还可以。 我去年申请过一支,曝光条带出来看着没问题,就是可能稀释的比例比较大才能爆出好的条带。但是这支抗体可能特异性没那么好。
Date published: 2017-10-15
Rated 5 out of 5 by from 非常完美的抗体 一个月前申请领用的,用的一抗浓度是1比500 ,效果非常好,希望后面的实验可以顺顺的进行下去,,,,,
Date published: 2017-09-14
Rated 5 out of 5 by from works in mouse liver I checked if the antibody works with mouse liver lysate. I can detect both p43 & p38 clearly with less back ground.
Date published: 2017-06-19
Rated 5 out of 5 by from strong signal The dilution 1:1000 been used, specific result and specific signal for WB analysis.
Date published: 2017-05-27
Rated 5 out of 5 by from good binding ablility i am happy to use this antibody, for an immune problem research. I use this antibody to detect the expression of dental derived Mesenchymal steme cells after some advert stimulation. I conducted the following procedures for WB: 1The cells were lysated with RIPA, PMSF, and proteinase cocktail ,then ultrasound 2 electrophoresis, transfer to NC membrane 3 incubated with first antibody sc-374615 is diluted at:1:150 ,4 °C,overnight (first time at 1:250, failed) 4 washed, incubated with goat anti-mouse HRP conjugate second antibody, ECL coloration 5 results good anyway ,the protein band is not bad
Date published: 2017-01-19
Rated 5 out of 5 by from Good for WB caspase-11 p20 Antibody (A-2) is an excellent choice for detection of mouse or rat in WB.
Date published: 2017-01-05
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