caspase-1 p20 Antibody (D-4) recommended for detection of caspase-1 precursor and p20 subunit of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA

caspase-1 p20 Antibody (D-4): sc-398715

caspase-1 p20 Antibody (D-4) is rated 4.0 out of 5 by 29.
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Datasheets

    • caspase-1 p20 Antibody (D-4) is a mouse monoclonal IgG2b κ, cited in 84 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 259-294 at the C-terminus of caspase-1 p20 of mouse origin
    • Anti-caspase-1 p20 Antibody (D-4) is recommended for detection of caspase-1 precursor and p20 subunit of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-caspase-1 p20 Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-1 p20 (D-4): sc-398715.
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for caspase-1 p20 Antibody (D-4) for WB and IHC(P) applications. This reagent is now offered in a bundle with caspase-1 p20 Antibody (D-4) (see ordering information below).
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What is the expected molecular weight of the protein detected by this antibody, caspase-1 p20 (D-4): sc-398715?

Asked by: TinTin
This antibody, caspase-1 p20: sc-398715, is recommended for the detection of caspase-1 precursor and p20 subunit. The expected molecular weights of these caspase forms are ~45kDa and ~20kDa, respectively, however detection of the p20 subunit can be difficult to observe by Western blot, so it is more common to only see the precursor band.
Answered by: Technical Support 12
Date published: 2017-01-24

What application is the blocking peptide sc-398715 P appropriate for?

Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Service 11
Date published: 2019-05-25
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Rated 5 out of 5 by from 条带清晰,背景底 我对这个抗体感到非常满意,价格优惠,希望santa cruz公司推出更多优秀的抗体,为全球科学家提供更多有价值的产品,做出更多的贡献。
Date published: 2021-12-22
Rated 5 out of 5 by from 功能出色 同事订购过此产品caspase-1 p20抗体 (D-4),使用效果不错,条带清晰,我希望可以获取小样试用,将会非常高兴。
Date published: 2018-05-11
Rated 5 out of 5 by from IHC Used it for IHC got very perfect staining for mouse colon section.1:150 very good concetration.
Date published: 2018-01-27
Rated 1 out of 5 by from Caspase-1 p20 antibody I received a sample size to test for active caspase-1 in the medium of BMDCs stimulated in various conditions. I wasn't able to detect any bands at all. :(
Date published: 2018-01-06
Rated 5 out of 5 by from Good! Caspase 1 p-20(d-4) protein: from mouse liver extracted with RIPA Loading 20ug/well
Date published: 2017-12-12
Rated 5 out of 5 by from excellent quality A good staining was obtained in rat colon tissue in IHC(1:100 dilution) and very good antibodies were obtained.
Date published: 2017-10-26
Rated 5 out of 5 by from 功能很好 上次申请的小样试用装非常好用,它不仅能曝出全长的caspase 1蛋白条带,还能曝出caspase-1 p20条带。但是就是还有些扎蛋白条带。不过都不影响条带。
Date published: 2017-10-15
Rated 3 out of 5 by from Trial Report sc-398715 I bought this antibody(sc-398715) a month ago and it's pity that there was no flaser. Maybe the protein I dectected was not form the model organism-mouse so there are many different amino acid sequences in this protein.
Date published: 2017-09-25
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