caspase-1 Antibody (D-3) recommended for detection of caspase-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

caspase-1 Antibody (D-3): sc-392736

caspase-1 Antibody (D-3) is rated 4.3 out of 5 by 18.
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Datasheets

    • caspase-1 Antibody (D-3) is a mouse monoclonal IgG1 κ caspase-1 antibody, cited in 49 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 367-391 near the C-terminus of caspase-1 of human origin
    • caspase-1 Antibody (D-3) is recommended for detection of caspase-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-caspase-1 Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-1 (D-3): sc-392736.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for caspase-1 Antibody (D-3) for WB and IHC(P) applications. These reagents are now offered in bundles with caspase-1 Antibody (D-3) (see ordering information below).
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what is the volume?

Asked by: ailin
Thank you for your question. The antibody comes as 200 µg in 1 ml.
Answered by: Tech Support Europe
Date published: 2021-08-12

Why do I always get a positive band at 72 kD?

Asked by: Lorrainedd
Thank you for your question. Please contact technical service for troubleshooting advice on your experiment.
Answered by: Tech Support Europe
Date published: 2022-07-30

What is the difference between D-3 and D-4? Do these detect cleaved caspase 1 as well?

Asked by: cindyha_uot
Thank you for your question. These are both mouse monoclonal antibodies, but they are have different isotypes, and are raised against different antigens. The caspase-1 Antibody (D-3): sc-392736 is raised against an epitope mapping between amino acids 367-391 (human origin), and caspase-1 p20 Antibody (D-4): sc-398715 is raised against an epitope mapping between amino acids 259-294 (mouse origin). Both antibodies are expected to detect cleaved caspase, however (D-3) should recognize the p10 subunit, and (D-4) should recognize the p20 subunit. Please contact our Technical Service Department for help in choosing the best antibody for your research.
Answered by: Technical Support
Date published: 2020-03-11

I was wondering how many tests can be run with one kit of the following Ab: caspase-1 Antibody (D-3): sc-392736

Asked by: Anonymous5
Thank you for your question. We provide 200 µg in 1 mL. The number of tests that can be performed using this antibody depends on your application, dilution and final working volumes.
Answered by: Technical Support
Date published: 2019-04-13

I used SC-1780 (caspase-1 Ab) to detect cleaved-caspase-1 (p20) polyclonal antibody. I would like to change the Ab to SC-392736 monoclonal Ab. But there is not any comment for detection of cleaved-caspase-1 (p20) protein. Can I detect using SC-392736?

Asked by: Jeong H
Thank you for your question. We would not recommend this mouse monoclonal, caspase-1 Antibody (D-3): sc-392736, for detection of the cleaved caspase-1 p20 subunit. This antibody is specific for an epitope mapping between amino acids 367-391 near the C-terminus of caspase-1 of human origin. And according to Uniprot, the p20 subunit corresponds to a different region between amino acids 120-297. http://www.uniprot.org/uniprot/P29466#ptm_processing
Answered by: Technical Support
Date published: 2022-08-07

What application is the blocking peptide sc-392736 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Didnt work for wetsern blot Didnt work for wetsern blot and there is a batch consistency with this antibody
Date published: 2019-12-04
Rated 4 out of 5 by from Good, decent antibody This antibody detected a strong 45 kDa band, but the background was too high. We even used 5% milk to block as instructed. Overall, the band could be seen even though high background.
Date published: 2018-03-14
Rated 5 out of 5 by from Worked well for western blot Detected good protein band at 1:200 dilution. no background or nonspecific bands
Date published: 2018-02-07
Rated 3 out of 5 by from Clear bands I saw clear bands at 48kda and 25kda however I was looking for bands at 45, 20, and 10. I'm not sure if I'll be able to use this antibody (using mouse lung homogenates).
Date published: 2017-11-30
Rated 4 out of 5 by from wb效果不错 我之前申请了caspase1抗体的试用装,感觉WB效果很好,但是稀释比例还需要进一步摸索,感觉还有变化的空间。
Date published: 2017-09-13
Rated 5 out of 5 by from perfect very nice,it work well in WB at 1:1000,rat platelet.
Date published: 2017-09-05
Rated 3 out of 5 by from Worked well for cell lysates (human) Detected procaspase 1 bands at 1:1000 dilution in 2% milk in TBST. The signal was week and lots of non specific bands were observed
Date published: 2017-08-15
Rated 5 out of 5 by from good result on WB The antibody caspase-1 works well in WB at 1:500, rat articular chondrocytes cells, protein bands are clear
Date published: 2017-08-10
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