caspase-1 Antibody (D-3)recommended for detection of caspase-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

caspase-1 Antibody (D-3): sc-392736

caspase-1 Antibody (D-3) is rated 4.4 out of 5 by 17.
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Datasheets

    • caspase-1 Antibody (D-3) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 367-391 near the C-terminus of caspase-1 of human origin
    • caspase-1 Antibody (D-3) is recommended for detection of caspase-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • caspase-1 Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-392736 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-1 (D-3): sc-392736.
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I use this product 1 year ago and I need to use it again. I need your recommendation for the protocol which can respond better by this situation. Thanks for your advice.

Asked by: soli1995
Thank you for your question. If stored properly at 4C, this antibody should still be working after 1 year. We do not characterize expiration dates for our antibodies, but we do warranty them for the recommended applications for one year from the date of purchase. Please contact our Technical Service Department for further support.
Answered by: Technical Support
Date published: 2019-07-30

I was wondering how many tests can be run with one kit of the following Ab: caspase-1 Antibody (D-3): sc-392736

Asked by: Anonymous5
Thank you for your question. We provide 200 µg in 1 mL. The number of tests that can be performed using this antibody depends on your application, dilution and final working volumes.
Answered by: Technical Support
Date published: 2019-04-13

I used SC-1780 (caspase-1 Ab) to detect cleaved-caspase-1 (p20) polyclonal antibody. I would like to change the Ab to SC-392736 monoclonal Ab. But there is not any comment for detection of cleaved-caspase-1 (p20) protein. Can I detect using SC-392736?

Asked by: Jeong H
Thank you for your question. We would not recommend this mouse monoclonal, caspase-1 Antibody (D-3): sc-392736, for detection of the cleaved caspase-1 p20 subunit. This antibody is specific for an epitope mapping between amino acids 367-391 near the C-terminus of caspase-1 of human origin. And according to Uniprot, the p20 subunit corresponds to a different region between amino acids 120-297. http://www.uniprot.org/uniprot/P29466#ptm_processing
Answered by: Technical Support
Date published: 2017-07-19

What application is the blocking peptide sc-392736 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good, decent antibody This antibody detected a strong 45 kDa band, but the background was too high. We even used 5% milk to block as instructed. Overall, the band could be seen even though high background.
Date published: 2018-03-14
Rated 5 out of 5 by from Worked well for western blot Detected good protein band at 1:200 dilution. no background or nonspecific bands
Date published: 2018-02-07
Rated 3 out of 5 by from Clear bands I saw clear bands at 48kda and 25kda however I was looking for bands at 45, 20, and 10. I'm not sure if I'll be able to use this antibody (using mouse lung homogenates).
Date published: 2017-11-30
Rated 4 out of 5 by from wb效果不错 我之前申请了caspase1抗体的试用装,感觉WB效果很好,但是稀释比例还需要进一步摸索,感觉还有变化的空间。
Date published: 2017-09-13
Rated 5 out of 5 by from perfect very nice,it work well in WB at 1:1000,rat platelet.
Date published: 2017-09-05
Rated 3 out of 5 by from Worked well for cell lysates (human) Detected procaspase 1 bands at 1:1000 dilution in 2% milk in TBST. The signal was week and lots of non specific bands were observed
Date published: 2017-08-15
Rated 5 out of 5 by from good result on WB The antibody caspase-1 works well in WB at 1:500, rat articular chondrocytes cells, protein bands are clear
Date published: 2017-08-10
Rated 4 out of 5 by from Good antibody We used under reducing and non-reducing conditions by WB and the results were specific and strong.
Date published: 2017-08-03
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