caspase-1 Antibody (14F468) recommended for detection of full-length caspase-1 and cleaved caspase-1 forms that retain amino acids 371-390 of the caspase-1 protein of mouse, rat, human and porcine origin by WB, IP, IF and IHC(P)

caspase-1 Antibody (14F468): sc-56036

caspase-1 Antibody (14F468) is rated 4.3 out of 5 by 42.
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Datasheets

    • caspase-1 Antibody (14F468) is a mouse monoclonal IgG1 κ, cited in 298 publications, provided at 100 µg/ml
    • raised against amino acids 371-390 of caspase-1 of human origin
    • recommended for detection of full-length caspase-1 and cleaved caspase-1 forms that retain amino acids 371-390 of the caspase-1 protein of mouse, rat, human and porcine origin by WB, IP, IF and IHC(P)
    • See caspase-1 (D-3): sc-392736 for caspase-1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-1 (14F468): sc-56036.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for caspase-1 Antibody (14F468) for WB and IHC(P) applications. These reagents are now offered in bundles with caspase-1 Antibody (14F468) (see ordering information below).
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will alexa fluor 488 or alexa fluor 555 work with this antibody while performing immunofluoroscence of mouse brain tissue?

Asked by: Adnan046
Thank you for your question . Both alexa flour 488 and 555 will work for immunofluoroscence with mouse tissue . Here is a list of secondary antibodies we offer : https://www.scbt.com/browse/Anti-Mouse-IgG-Binding-Protein-Secondaries/_/N-130b1ry.
Answered by: BlakeJ
Date published: 2022-11-17

Hi, I have a question, how many band are revealed with Casp1 antibody (sc-56036)? after my treatment I saw  3 bands (45kDa; 35kDa; 25-27kda) best regards Vincenzo

Asked by: vincenzo81
Thank you for your question. Anti-caspase-1 Antibody (14F468): sc-56036 was raised against amino acids 371-390 of caspase-1 of human origin. As such it is able to recognize any of the five described isoforms for this protein. The bands you are detecting correspond to isoforms alpha, gamma, and delta.
Answered by: Tech Support Europe
Date published: 2022-02-04

What concentration of stimulated THP-1 cells were used for the image on the datasheet?

Asked by: AnonymousII
Thank you for your question. Following our standard protocol, 40–60 µg whole cell lysate of THP-1 cells were used for these western blots. You can find our recommended protocol for western blotting by following this link: https://www.scbt.com/de/resources/protocols/western-immuno-blotting
Answered by: Tech Support Europe
Date published: 2020-06-23

What are the conditions to stimulate THP-1 cells with PMA and LPS to detect CASP1 p10, similar the image of the datasheet? What is the PMA and LPS concentration? How much time?

Asked by: Anonymous
Thank you for your question. Here are the conditions we suggest for stimulation using LPS and PMA, respectively: 5 µg per mL of LPS, induction time 2 hours. 40ng/ml of PMA, induction time 2 hours.
Answered by: Tech Service
Date published: 2020-02-05

I wonder why we have multibanding for 45kDa. (I work with placental tissue). If it considered good? Why we have these multiple bads?

Asked by: Anna Anna
Thank you for your question. The expected molecular weight for this protein is ~45kDa, but we do not have any information regarding other sized bands. Please contact our Technical Service Department or your local distributor for assistance in troubleshooting this antibody.
Answered by: Tech Service
Date published: 2019-06-19

We worked with your caspase-1 p10 (M-20) ab previously, which has been now discontinued. You have indicated to use sc-56036 ab (above). We have bought the antibody and tried it with no luck. Can you please advise?

Asked by: Dendritic cells
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-06-19

你好!这个抗体染出来有两个条带吗?上面是Pro-caspase-1,下面是Caspase-1,是这样吗?谢谢!

Asked by: July Wang
感谢您的提问。关于此问题的详细细节,欢迎通过:电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. For more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2019-07-08

Greetings, we are very interest in your anti-mouse caspase 1 antibody and would like the trial sample to test in our cell culture model. What is the best way in obtaining a free sample to test. Best regards, Giang

Asked by: Giang
Thank you for your question. Please contact our Technical support group by phone, email ([email protected]) or via live chat on our website, www.scbt.com, and we would be happy to assist you with a sample for caspase-1.
Answered by: Tech Service
Date published: 2017-12-12
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Rated 5 out of 5 by from nice very nice,it work well in WB.Good specificity,no background
Date published: 2021-07-31
Rated 4 out of 5 by from WB的条带很明显 这个抗体的前体条带特别的明显,但是剪切的条带相对来说比较弱,在不同细胞上都有检测,效果还可以!!!!
Date published: 2020-12-31
Rated 5 out of 5 by from Excellent antibody It shows excellent results in western blot, with both pro-caspase-1 and cleaved caspase-1 detected.
Date published: 2020-09-01
Rated 5 out of 5 by from wnt-1抗体非常不错 通过代理拿到了wnt-1抗体小样,WB非常不错,条带清晰,没有干扰的咋带。考虑下游通路继续用该公司抗体。
Date published: 2020-07-10
Rated 4 out of 5 by from good I hope that the product will continue to work well, and the experiment will depend on you later.
Date published: 2019-07-09
Rated 5 out of 5 by from great antibody I received this as a sample last year, our group tried this antibody on pig lung samples. it worked well for us.. thanks a lot.
Date published: 2018-05-10
Rated 2 out of 5 by from western blotting-no cleaved band! Used this antibody (1:200 dilution) for western blotting of whole AC16 cell lysates. Only one band (pro-caspase-1) was visible at the expected molecular weight. There were no traces of the mature p10 band even after using a stronger developer with longer exposure time.
Date published: 2018-04-27
Rated 1 out of 5 by from Multile non specific bands with new lot We ordered this antibody and received lot.no: B2318. The antibody with this lot number is not at all good.It gives multiple bands. The antibody with lot number A0517 was extremely good.
Date published: 2018-04-05
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