caspase-1 Antibody (14F468) recommended for detection of full-length caspase-1 and cleaved caspase-1 forms that retain amino acids 371-390 of the caspase-1 protein of mouse, rat, human and porcine origin by WB, IP, IF and IHC(P)

caspase-1 Antibody (14F468): sc-56036

caspase-1 Antibody (14F468) is rated 4.3 out of 5 by 39.
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Datasheets

    • caspase-1 Antibody (14F468) is a mouse monoclonal IgG1 (kappa light chain) provided at 100 µg/ml
    • raised against amino acids 371-390 of caspase-1 of human origin
    • recommended for detection of full-length caspase-1 and cleaved caspase-1 forms that retain amino acids 371-390 of the caspase-1 protein of mouse, rat, human and porcine origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

What concentration of stimulated THP-1 cells were used for the image on the datasheet?

Asked by: AnonymousII
Thank you for your question. Following our standard protocol, 40–60 µg whole cell lysate of THP-1 cells were used for these western blots. You can find our recommended protocol for western blotting by following this link: https://www.scbt.com/de/resources/protocols/western-immuno-blotting
Answered by: Tech Support Europe
Date published: 2020-06-23

What are the conditions to stimulate THP-1 cells with PMA and LPS to detect CASP1 p10, similar the image of the datasheet? What is the PMA and LPS concentration? How much time?

Asked by: Anonymous
Thank you for your question. Here are the conditions we suggest for stimulation using LPS and PMA, respectively: 5 µg per mL of LPS, induction time 2 hours. 40ng/ml of PMA, induction time 2 hours.
Answered by: Tech Service
Date published: 2020-02-05

I wonder why we have multibanding for 45kDa. (I work with placental tissue). If it considered good? Why we have these multiple bads?

Asked by: Anna Anna
Thank you for your question. The expected molecular weight for this protein is ~45kDa, but we do not have any information regarding other sized bands. Please contact our Technical Service Department or your local distributor for assistance in troubleshooting this antibody.
Answered by: Tech Service
Date published: 2019-06-19

We worked with your caspase-1 p10 (M-20) ab previously, which has been now discontinued. You have indicated to use sc-56036 ab (above). We have bought the antibody and tried it with no luck. Can you please advise?

Asked by: Dendritic cells
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-06-19

你好!这个抗体染出来有两个条带吗?上面是Pro-caspase-1,下面是Caspase-1,是这样吗?谢谢!

Asked by: July Wang
感谢您的提问。关于此问题的详细细节,欢迎通过:电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. For more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2019-07-08

Greetings, we are very interest in your anti-mouse caspase 1 antibody and would like the trial sample to test in our cell culture model. What is the best way in obtaining a free sample to test. Best regards, Giang

Asked by: Giang
Thank you for your question. Please contact our Technical support group by phone, email ([email protected]) or via live chat on our website, www.scbt.com, and we would be happy to assist you with a sample for caspase-1.
Answered by: Tech Service
Date published: 2017-12-12

Será possível obter uma amostra gratuita para fazer um estudo relacionado com a IL-1b? O meu laboratório já comprou este anticorpo mas deixou de funcionar por algum motivo

Asked by: Leonor Rodrigues
Thank you for your question. Please contact our Technical Service Department by phone or e-mail with your shipping address and with the catalogue number requested as sample.
Answered by: Technical Support Europe
Date published: 2017-10-06

Should i use 5% w/v BSA or nonfat dry milk for dilution of Antibody for western blot application?

Asked by: JuliaSt
Thank you for your question. We recommend using 5% nonfat milk + TBST for blocking and dilution of both primary and secondary antibodies for Western blotting with this antibody, caspase-1 (14F468): sc-56036. This blocking buffer is available pre-made (TBS Blotto A: sc-2333). A link to our recommended protocol is below as a reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-08-01
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Rated 5 out of 5 by from wnt-1抗体非常不错 通过代理拿到了wnt-1抗体小样,WB非常不错,条带清晰,没有干扰的咋带。考虑下游通路继续用该公司抗体。
Date published: 2020-07-10
Rated 4 out of 5 by from good I hope that the product will continue to work well, and the experiment will depend on you later.
Date published: 2019-07-09
Rated 5 out of 5 by from great antibody I received this as a sample last year, our group tried this antibody on pig lung samples. it worked well for us.. thanks a lot.
Date published: 2018-05-10
Rated 2 out of 5 by from western blotting-no cleaved band! Used this antibody (1:200 dilution) for western blotting of whole AC16 cell lysates. Only one band (pro-caspase-1) was visible at the expected molecular weight. There were no traces of the mature p10 band even after using a stronger developer with longer exposure time.
Date published: 2018-04-27
Rated 1 out of 5 by from Multile non specific bands with new lot We ordered this antibody and received lot.no: B2318. The antibody with this lot number is not at all good.It gives multiple bands. The antibody with lot number A0517 was extremely good.
Date published: 2018-04-05
Rated 4 out of 5 by from 抗体还不错 提取的组织样本做western blot,上样量50微克,效果如图,偶有非特异性条带,主要条带还是比较清晰的。
Date published: 2018-01-26
Rated 5 out of 5 by from 质量很好 我一个月之前买的,用的还不错,用的还不错,质量高,质量高,谢谢你们的支持,谢谢你们的支持......
Date published: 2017-10-30
Rated 5 out of 5 by from Great IF Staining This antibody works extremely well with IF in mice brain.
Date published: 2017-09-27
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