Anti-casein kinase II beta Antibody (E-9) recommended for detection of casein kinase IIβ of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA

Anti-casein kinase IIβ Antibody (E-9): sc-46666

Anti-casein kinase IIβ Antibody (E-9) is rated 5.0 out of 5 by 3.
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Datasheets

    • casein kinase IIβ Antibody (E-9) is a mouse monoclonal IgG1 κ, cited in 11 publications, provided at 200 µg/ml
    • raised against amino acids 1-215 representing full length casein kinase IIβ of human origin
    • recommended for detection of casein kinase IIβ of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
    • See casein kinase IIβ (6D5): sc-12739 for casein kinase IIβ antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for casein kinase IIβ Antibody (E-9) for WB and IHC(P) applications. These reagents are now offered in bundles with casein kinase IIβ Antibody (E-9) (see ordering information below).
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I am using casein kinase IIβ (E-9): sc-46666 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Produced superb immunoperoxidase nuclear Produced superb immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human thyroid gland tissue. -SCBT QC
Date published: 2015-05-19
Rated 5 out of 5 by from Strong band in WB in HepG2 Strong band in WB in HepG2, K-562 and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-03-29
Rated 5 out of 5 by from Positive signal in WB in mouse transfected Positive signal in WB in mouse transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2015-02-09
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