Anti-casein kinase II alpha Antibody (F-10) recommended for detection of casein kinase IIα and casein kinase IIα′ of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA

Anti-casein kinase IIα Antibody (F-10): sc-365763

Anti-casein kinase IIα Antibody (F-10) is rated 5.0 out of 5 by 2.
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Datasheets

    • casein kinase IIα Antibody (F-10) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 1-320 of casein kinase IIα of human origin
    • recommended for detection of casein kinase IIα and casein kinase IIα′ of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine, bovine, porcine and avian
    • See casein kinase IIα (E-7): sc-373894 for casein kinase IIα antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for casein kinase IIα Antibody (F-10). This work is in progress.
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Can casein kinase IIα (F-10): sc-365763 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Trav11
Thank you for your question. Yes, casein kinase IIα (F-10): sc-365763 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Satisfactory Western Blot data of casein Satisfactory Western Blot data of casein kinase II expression in HeLa, NIH/3T3 and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-04-13
Rated 5 out of 5 by from Satisfactory Western Blot data of casein Satisfactory Western Blot data of casein kinase II expression in K-562 and Jurkat nuclear extracts and K-562, MOLT-4, MCF7 and Hep G2 whole cell lysates. -SCBT QC
Date published: 2013-11-11
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