Anti-casein kinase II alpha Antibody (E-7) recommended for detection of casein kinase IIα of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA

Anti-casein kinase IIα Antibody (E-7): sc-373894

Anti-casein kinase IIα Antibody (E-7) is rated 5.0 out of 5 by 10.
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Datasheets

    • Anti-casein kinase IIα Antibody (E-7) is a mouse monoclonal IgG2a κ casein kinase IIα antibody, cited in 9 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 2-27 at the N-terminus of casein kinase IIα of human origin
    • casein kinase II alpha Antibody (E-7) is recommended for detection of casein kinase IIα of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine, bovine, porcine and avian
    • Anti-casein kinase II alpha Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of casein kinase IIα (E-7): sc-373894.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for casein kinase IIα Antibody (E-7) for WB and IHC(P) applications. These reagents are now offered in bundles with casein kinase IIα Antibody (E-7) (see ordering information below).
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What application is the blocking peptide sc-373894 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great of IF and WB We have been really happy with this antibody. It works great for WB at a 1:200 dilution in milk - I have even used this in 5%BSA/1XPBST and used repeatedly overnight at 4C - it can work for 3-4 uses, especially if you maintain at 4C by performing primary incubation overnight. Also works great for IF at a 1:100 dilution
Date published: 2019-04-29
Rated 5 out of 5 by from Very good antibody Works very well on IF and western blot. Specificity tested with siRNA against CK2 alpha.
Date published: 2018-10-01
Rated 5 out of 5 by from 발현이 잘 됩니다 샘플로 먼저 받아서 사용해 봤는데 제가 실험하고 있는 유방암 세포주에서 발현이 잘 됩니다.
Date published: 2018-07-24
Rated 5 out of 5 by from Perfect detection on Western Blot! I tested this antibody by Western Blot using total protein extracts from human primary fibroblasts. It showed great sensitivity and specificity!
Date published: 2017-10-02
Rated 5 out of 5 by from Excellent WB bands! Strong and clear WB bands in mouse cardiomyocytes and mouse cardiac tissue!
Date published: 2017-06-28
Rated 5 out of 5 by from good bands I got it one week ago ,detect mice liver protein and got single band,very good
Date published: 2017-05-25
Rated 5 out of 5 by from good production The product quality is very good, and result of the band is very clear and strong even at a 1:800 dilution in western blot
Date published: 2017-05-01
Rated 5 out of 5 by from Highly potent and specific antibody This monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was extremely strong detection of the ~42 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested, except for a medium cross-reactive 55 kDa protein.
Date published: 2016-11-10
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