Anti-casein kinase II alpha / alpha ' Antibody (31) recommended for detection of casein kinase IIα and casein kinase IIα′ of mouse, rat and human origin by WB and IP

Anti-casein kinase IIα/α' Antibody (31): sc-136281

Anti-casein kinase IIα/α' Antibody (31) is rated 4.0 out of 5 by 1.
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Datasheets

    • casein kinase IIα/α' Antibody (31) is a mouse monoclonal IgG1, cited in 2 publications, provided at 50 µg/0.5 ml
    • raised against amino acids 1-123 of casein kinase IIα of rat origin
    • recommended for detection of casein kinase IIα and casein kinase IIα′ of mouse, rat and human origin by WB and IP
    • See casein kinase IIα (E-7): sc-373894 for casein kinase IIα/α' antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for casein kinase IIα/α' Antibody (31). This work is in progress.
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I am using casein kinase IIα/α' (31): sc-136281 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Professor Griffin
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Positive signal in WB in rat cerebellum tissue Positive signal in WB in rat cerebellum tissue extract. -SCBT QC
Date published: 2013-09-08
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