CAPSOA buffer salt used in the electrotransfer of proteins to nitrocellulose membranes

CAPSO (CAS 73463-39-5)

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Application: A buffer salt used in the electrotransfer of proteins to nitrocellulose membranes
CAS Number: 73463-39-5
Purity: ≥97%
Molecular Weight: 237.32
Molecular Formula: C9H19NO4S
* Refer to Certificate of Analysis for lot specific data (including water content).
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CAPSO (N-cyclohexyl-2-hydroxyl-3-aminopropanesulfonic acid) buffer salt is used to formulate CAPSO buffer, a zwitterionic buffer that is useful in the range of pH 8.9 - 10.3. CAPSO buffer is used in western and immunoblotting experiments as well as protein sequencing and identification. Used in the electrotransfer of proteins to PVDF (sc-3723) or nitrocellulose membranes (sc-3718, sc-3724). The high pH of this buffer makes it useful for the transfer of proteins with a pI > 8.5. CAPSO is not one of the original Good′s buffers, although it has a similar structure to the other propanesulfonic acids and was selected as a highly water soluble buffering reagent with an optimum buffering pH of 10.4 and minimal reactivity with enzymes or proteins, minimal salt effects.
Also available:
CAPS (sc-216090)
CAPSO sodium salt(sc-285280)


References

1. Oberley, T.D., et al. 1983. Exp. Cell Res. 145: 265-276. PMID: 6861897
2. Kaushal, V. and Barnes, L.D. 1986. Anal. Biochem. 157: 291-294. PMID: 3777432
3. Wiedemeyer, R., et al. 2003. Oncogene. 22: 401-411. PMID: 12545161
4. Zaitseva, J., et al. 2004. Acta Crystallogr. D Biol. Crystallogr. 60: 1076-1084. PMID: 15159567
5. Striegler, S., et al. 2010. Inorg Chem. 49: 2639-2648. PMID: 20166683

Appearance :
Crystalline powder
Physical State :
Solid
Solubility :
Soluble in water (10 g+ 90 ml).
pH :
Useful pH range 8.9 – 10.3
Storage :
Store at room temperature
Melting Point :
270-274° C
Density :
1.30 g/cm3
Refractive Index :
n20D 1.54
pK Values :
pKa: 9.6 at 25° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
2733480
MDL Number :
MFCD00041778
Beilstein Registry :
5939234
SMILES :
C1CCC(CC1)NCC(CS(=O)(=O)O)O

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Certificate of Analysis

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CAPSO  Product Citations

See how others have used CAPSO. Click on the entry to view the PubMed entry .

Citations 1 to 2 of 2 total

PMID: # 16687400  Radchenko, MV. et al. 2006. J. Biol. Chem. 281: 19822-19829.

PMID: # 12547799  Váró, G. et al. 2003. Biophys. J. 84: 1202-1207.

Citations 1 to 2 of 2 total
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