CAP2 Antibody (NN12)recommended for detection of CAP2 of human origin by WB, IP, IF and ELISA

CAP2 Antibody (NN12): sc-100916

CAP2 Antibody (NN12) is rated 5.0 out of 5 by 2.
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Datasheets

    • CAP2 Antibody (NN12) is a mouse monoclonal IgG1 (kappa light chain) provided at 100 µg/ml
    • raised against recombinant CAP2 of human origin
    • recommended for detection of CAP2 of human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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I am using CAP2 (NN12): sc-100916 for immunofluorescence, what dilution of this antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Produced beautiful immunofluorescence membrane Produced beautiful immunofluorescence membrane and cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-04-18
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