CAP2 Antibody (A-5)recommended for detection of CAP2 of mouse, rat and human origin by WB, IP, IF and ELISA

CAP2 Antibody (A-5): sc-377471

CAP2 Antibody (A-5) is rated 5.0 out of 5 by 3.
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Datasheets

    • CAP2 Antibody (A-5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 77-121 mapping near the N-terminus of CAP2 of human origin
    • CAP2 Antibody (A-5) is recommended for detection of CAP2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • CAP2 Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CAP2 (A-5): sc-377471.
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What is the recommended fixation method for immunofluorescence staining with CAP2 (A-5): sc-377471 monoclonal antibody?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from nice product! dilution rate 1:300 for WB can get a clear and single band in SW480,SW48,HCT116,HT29,LOVO,DLD1.
Date published: 2017-09-12
Rated 5 out of 5 by from Produced beautiful immunofluorescence cytoplasmic Produced beautiful immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-03
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