CaMKK beta Antibody (C-11)recommended for detection of CaMKKβ of human origin by WB, IP, IF and ELISA

CaMKKβ Antibody (C-11): sc-271674

CaMKKβ Antibody (C-11) is rated 4.6 out of 5 by 7.
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Datasheets

    • CaMKKβ Antibody (C-11) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-95 mapping at the N-terminus of CaMKKβ of human origin
    • CaMKK beta Antibody (C-11) is recommended for detection of CaMKKβ of human origin by WB, IP, IF and ELISA
    • CaMKK beta Antibody (C-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CaMKKβ (C-11): sc-271674.
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What blocking solution should I use with CaMKKβ (C-11): sc-271674 monoclonal antibody for Western blot?

Asked by: jenni
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335) Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Santa Cruz TS
Date published: 2017-01-31
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Rated 3 out of 5 by from Works in WB. Human MSCs Weak band can be detected by Western blot in human MSCs.
Date published: 2017-09-18
Rated 5 out of 5 by from good signal result The antibody been tested in Human breast cell lines, there was specific signal at 66kda,after,incubate in RT for 1 hour, the band is very strong even diluted 1:1000,after long time exposure 1hour,there was a little background,so I recommend exposed short time.
Date published: 2017-07-23
Rated 5 out of 5 by from strong signal for WB good quality antibody and clear signal, no background, the band is very clean for WB.
Date published: 2017-06-03
Rated 5 out of 5 by from Good Western Blot Results WE used this antibody in a western blot for human samples and got a clear distinct band
Date published: 2016-12-22
Rated 5 out of 5 by from I got results I got results, it worked well, no issues, 5 stars
Date published: 2015-04-07
Rated 5 out of 5 by from Worked well for WB in H4 Worked well for WB in H4, SK-N-SH, T98G, and U-87 MG whole cell lysates. -SCBT QC
Date published: 2015-02-18
Rated 4 out of 5 by from Robust band in WB in IMR Robust band in WB in IMR-32 whole cell lysate. -SCBT QC
Date published: 2014-01-04
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