CaMKIV CRISPR Plasmids (h)human CaMKIV-specific CRISPR/Cas9 KO Plasmid, HDR Plasmid, Double Nickase Plasmids, CRISPR Activation Plasmids and Lentiviral Activation Particles

CaMKIV CRISPR Plasmids (h)

CaMKIV CRISPR Knockout and Activation Products (h) is rated 5.0 out of 5 by 1.
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Datasheets

    • Target species: human; for corresponding mouse product, see CaMKIV CRISPR Plasmids (m)
    • CRISPR/Cas9 KO Plasmids consists of CaMKIV-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library
    • For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
    • Target-specific CRISPR Plasmids for both gene knockout and activation are available. Please refer to the detailed product information in the tabs below
    • Gene knockdown or activation can be assayed using CaMKIV Antibody (H-5): sc-55501
    • All products are provided as transfection-ready, purified plasmid DNA, except the Lentiviral Activation Particles which have been prepackaged as Lentiviral Particles for use with hard-to-transfect cells
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Gene Info

SpeciesGene NameGene IDChromosome LocationIsoform (mRNA) Accession #Protein Accession #OMIM™ Number
HumanCAMK48145q22.1NM_001323374, NM_001323375, NM_001323376, NM_001323377, NM_001744Q16566114080
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Rated 5 out of 5 by from Excellent design, 100% works! I previously tried CaMKK2 CRISP/cas9 combined with HDR plasmids and got excellent result in knocking out the gene. This time I wanted to knockout CaMK4 and thought that I just use the CRISPR/cas9 constructs because the selected clones will not have the RFP/puromycin that you get when use HDR plasmids. So, my strategy was to transfect the cells with cocktail of 3 gRNA Cas9 plasmids (NHEJ based gene editing) and then FACS sort the GFP positive cell (see the attached picture), the FACS sorted GFP positive cells were then plated at a density of one cell per well in 96 well plates and the clones were expanded and total proteins were subjected to immunoblotting using anti-CaMK4 and GAPDH antibodies from SCBT (fabulous antibodies, see the blot picture). As you can see in the attached picture that I got knockout clones at very high rate and the best part is that I used minimum effort to get my knockout cell line within a month. So, all credit goes to the beautiful gRNA designed by SCBT scientist. Use it and make exciting discoveries.
Date published: 2017-09-07
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