CaMKIV Antibody (H-5)recommended for detection of CaMKIV of mouse, rat and human origin by WB, IP, IF and ELISA

CaMKIV Antibody (H-5): sc-55501

CaMKIV Antibody (H-5) is rated 4.6 out of 5 by 7.
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Datasheets

    • CaMKIV Antibody (H-5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 328-473 mapping at the C-terminus of CaMKIV of mouse origin
    • CaMKIV Antibody (H-5) is recommended for detection of CaMKIV of mouse, rat and human origin by WB, IP, IF and ELISA
    • CaMKIV Antibody (H-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CaMKIV (H-5): sc-55501.
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What is the recommended fixation method for immunofluorescence staining with CaMKIV (H-5): sc-55501 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from 免疫染色 マウス単離細胞に対して蛍光免疫染色を行いました。多少ノンスペがありましたが、良好な染色画像を得ました。
Date published: 2018-11-12
Rated 4 out of 5 by from Good Antibody I tested this antibody on mouse brain lysate, detected single protein band in WB (1:200 dilution).
Date published: 2018-07-09
Rated 5 out of 5 by from what we need The main ingredient of this product, CaMKII, is a widely expressed serine / threonine protein kinase, which is involved in the regulation of cell cycle and transcription by calmodulin activation. We think that this feature is what we need
Date published: 2017-02-09
Rated 5 out of 5 by from Great antibody CaMKIV Antibody (H-5) produced nice clear bands and beautiful staining by IHCP and IF
Date published: 2017-01-25
Rated 5 out of 5 by from Good band in WB in human CaMKIV transfected Good band in WB in human CaMKIV transfected 293 whole cell lysate. -SCBT QC
Date published: 2015-03-11
Rated 4 out of 5 by from Large band seen in WB in human CaMKIV transfected Large band seen in WB in human CaMKIV transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-01-17
Rated 5 out of 5 by from Great specificity in WB in Jurkat whole cell Great specificity in WB in Jurkat whole cell lysate. -SCBT QC
Date published: 2013-05-09
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