CaMKIV Antibody (A-3)recommended for detection of CaMKIV and Calspermin of human origin by WB, IP, IF, IHC(P) and ELISA

CaMKIV Antibody (A-3): sc-166156

CaMKIV Antibody (A-3) is rated 5.0 out of 5 by 6.
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Datasheets

    • CaMKIV Antibody (A-3) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 423-461 at the C-terminus of CaMKIV of human origin
    • CaMKIV Antibody (A-3) is recommended for detection of CaMKIV and Calspermin of human origin by WB, IP, IF, IHC(P) and ELISA
    • CaMKIV Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-166156 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CaMKIV (A-3): sc-166156.
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What application is the blocking peptide sc-166156 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Excellent antibody: All in One! CaMK-IV (SC166156) is an excellent antibody and it will help you to paint your blot with clear band as well as spot the protein in the crowded intra-cellular environment (Immuno-fluorescence detection). I just purchased this antibody to use it for immunoblot and for Immuno-fluorescence study in human cell line. In Immunoblot it gives a clear band at 55kDa which is the right molecular weight and match perfectly with theoretical mol. weight prediction (see the attached blots). However, do not get disappointed if you see occasional high mol. weight band (as in the 2nd blot), which definitely is post translational modification (can't disclose further) and if you explore it may lead to great discovery. In immuno-fluorescence it showed clear localization in the cytoplasm, as well as in the nucleus and nucleolus, the nucleolar localization is very distinct and other scientist reported this. So if you are looking for a multipurpose CaMK-IV antibody then this is the ONE. Go for it and do great science.
Date published: 2017-05-05
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmic Produced beautiful immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human duodenum tissue. -SCBT QC
Date published: 2015-03-13
Rated 5 out of 5 by from Good specificity in WB in 293T whole cell lysate Good specificity in WB in 293T whole cell lysate. -SCBT QC
Date published: 2015-03-07
Rated 5 out of 5 by from Strong band in WB in human CaMKIV transfected Strong band in WB in human CaMKIV transfected 293 whole cell lysate. -SCBT QC
Date published: 2015-03-03
Rated 5 out of 5 by from Good Good, specific band seen in WB in Ramos and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-03-02
Rated 5 out of 5 by from Strong band in WB in human CaMKIV transfected Strong band in WB in human CaMKIV transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-02-17
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