CaMKII alpha Antibody (6G9)recommended for detection of CaMKIIα of mouse, rat and human origin by WB, IP, IF and IHC(P)

CaMKIIα Antibody (6G9): sc-32288

CaMKIIα Antibody (6G9) is rated 4.3 out of 5 by 7.
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Datasheets

    • CaMKIIα Antibody (6G9) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against partially purified CaM kinase II of rat origin
    • CaMKII alpha Antibody (6G9) is recommended for detection of CaMKIIα of mouse, rat and human origin by WB, IP, IF and IHC(P); non cross-reactive with 60 kDa β subunit in either phosphorylation site
    • CaMKII alpha Antibody (6G9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CaMKIIα (6G9): sc-32288.
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Hola! ¿Me podrían decir por favor frente a que región está dirigido este anticuerpo CaMKIIalfa (6G9)? Muchas gracias.

Asked by: RosP
Gracias por su pregunta. No hemos caracterizado el sitio de unión exacto de CaMKIIα (6G9): sc-32288. Sin embargo, se sabe que reacciona con la subunidad alfa tanto fosforilada como no fosforilada de CaM kinase II. Thank you for your question. We have not characterized the exact binding site of CaMKIIα (6G9): sc-32288. However, it is known to react with both phosphorylated and non-phosphorylated alpha subunit of CaM kinase II.
Answered by: Technical Support
Date published: 2018-12-31

I am using CaMKIIα (6G9): sc-32288 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Ab funziona bene! Ho testato l'anticorpo (dil. 1:500 come da datasheet, ma potrebbe essere benissimo usato 1:1000) su un lisato proteico di cervelletto murino. L'anticorpo ha riconosciuto in maniera specifica due bande molto vicine (un doppietto) a circa 45-47kDa.
Date published: 2019-03-22
Rated 5 out of 5 by from Excellent Antibody I tested this antibody on mouse brain lysate, detected a single strong sign
Date published: 2018-07-06
Rated 5 out of 5 by from Great for IHC(P) and WB CaMKIIα (6G9) gave a really nice band at 50 kDa in mouse cerebellum extract: sc-2403.
Date published: 2017-02-03
Rated 4 out of 5 by from We got good staining in IF in mouse cells We got good staining in IF in mouse cells with this antibody.
Date published: 2015-09-14
Rated 4 out of 5 by from Published data for WB and IF in monkey brain Published data for WB and IF in monkey brain sample. -SCBT Publication Review
Date published: 2015-04-28
Rated 4 out of 5 by from Clear band in WB in mouse brain and rat brain Clear band in WB in mouse brain and rat brain tissue extracts. -SCBT QC
Date published: 2015-02-24
Rated 4 out of 5 by from Published data for WB in mouse Cerebellar Published data for WB in mouse Cerebellar Purkinje cells. -SCBT Publication Review
Date published: 2015-01-23
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