CaMKII Antibody (G-1)recommended for detection of CaMKIIα, CaMKIIβ, CaMKIIγ and CaMKIIδ subunits of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

CaMKII Antibody (G-1): sc-5306

CaMKII Antibody (G-1) is rated 4.2 out of 5 by 13.
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Datasheets

    • CaMKII Antibody (G-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 303-478 of CaMKIIα of mouse origin
    • CaMKII Antibody (G-1) is recommended for detection of CaMKIIα, CaMKIIβ, CaMKIIγ and CaMKIIδ subunits of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • CaMKII Antibody (G-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CaMKII (G-1): sc-5306.
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It would be great if I could get a free 10 µg sample of CaMKII (G-1): sc-5306. Thanks

Asked by: PARU
Please, send us an email to uk@scbt.com with your complete delivery address and contact information. We don't have currently a limit in the samples we can send per order, would you, or any of your colleagues, like to try any other antibody? please let us know so we make a single shipment.
Answered by: Technical Support Europe
Date published: 2017-07-18

Can this antibody be used in IHC with FFPE?

Asked by: AbPolly
Thank you for your inquiry. Yes, our CaMKII antibody sc-5306 is validated for use in IHC with formalin fixed, paraffin-embedded tissue sections. We recommend performing antigen retrieval with sodium citrate buffer, pH 6, and heat. The complete protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 13
Date published: 2017-01-04
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Rated 4 out of 5 by from Immunostaining for cortical neurons from mouse I conducted immunocytostainig using mouse cortical neurons. This ntibody works well in 1:500 dilution.
Date published: 2019-09-09
Rated 5 out of 5 by from Great Clear multiple bands show different isoforms correctly.
Date published: 2017-11-15
Rated 4 out of 5 by from 试用装还可以 申请的试用装,在小鼠神经干细胞(NE4C细胞系),做的WB,1:1000条带挺清楚的,性价比应该是比较高的
Date published: 2017-10-14
Rated 5 out of 5 by from good This antibody is reactive with mouse, rat, and human. It will provide a great stain for any of those samples. If you are performing any IHC, then this will give you a great WB as well. It comes with several citations.
Date published: 2017-09-27
Rated 1 out of 5 by from Does not seem to work in zebra finch brain CaMKII antibody does not seem to be specific to neurons in the zebra finch brain. Has not been tested in concentration higher than 1:500. Details: 4% paraformaldehyde-perfused zebra finch; Permeabilization: 0.3% Triton-X; CaMKII antibody at 1:500.
Date published: 2017-09-15
Rated 4 out of 5 by from The specificity is not very good . This antibody has been used many times before feeling that the background is not very clean and that it is still useful to know if the tape is closed and that the time required for TBST washing needs to be changed. And with a heavy shadow, but with the instructions on the two strip is not the same, but not yet clear what is the case .
Date published: 2017-08-14
Rated 1 out of 5 by from Didnot work for WB No bands were detected using MCF-7 and MDA-MB-231 cell lysates. Used the ab at 1:1000 dilution.
Date published: 2017-07-03
Rated 5 out of 5 by from clear staining of cell bodies and dendrites The antibody was tested in 4% PFA perfusion fixed frozen sections of rat brain. Tested at dilutions 1/200, 1/800, 1/1600, 1/3200 and 1/12800. 1/1600 found to be optimal. Also tested at dilution 1/800 with permeabilisation using 0.2% Triton X-100 in the buffer, but this did not enhance results. A quench for endogenous peroxidase was included prior to the blocking step (0.3% H2O2 in PBS) Testing conducted at room temperature throughout. Good results obtained - clear staining of cell bodies and dendrites. Little staining in antibody-free control. The primary incubation was about 44hours - over 2 nights at room temp - started about 1pm on the first day and next stage started about 9am on the third day. We may be able to supply a photo in due course.
Date published: 2017-02-17
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