calsequestrin 1 Antibody (D-10)recommended for detection of calsequestrin 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

calsequestrin 1 Antibody (D-10): sc-137080

calsequestrin 1 Antibody (D-10) is rated 5.0 out of 5 by 3.
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Datasheets

    • calsequestrin 1 Antibody (D-10) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 31-90 mapping near the N-terminus of calsequestrin 1 of human origin
    • calsequestrin 1 Antibody (D-10) is recommended for detection of calsequestrin 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • calsequestrin 1 Antibody (D-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of calsequestrin 1 (D-10): sc-137080.
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I am using calsequestrin 1 (D-10): sc-137080 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Strong western blot signal A good western blot result was obtained - strong signal with no background with this anti-CASQ1 antibody. Sample: HEK293T overexpression cell lysates.
Date published: 2016-12-06
Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmic Produced superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human skeletal muscle tissue. -SCBT QC
Date published: 2015-01-28
Rated 5 out of 5 by from Produced positive Western Blot data of human Produced positive Western Blot data of human recombinant calsequestrin 1 fusion protein. -SCBT QC
Date published: 2013-12-17
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