Calretinin Antibody (34)recommended for detection of Calretinin of mouse, rat and human origin by WB, IP and IF

Calretinin Antibody (34): sc-135853

Calretinin Antibody (34) is rated 5.0 out of 5 by 3.
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Datasheets

    • Calretinin Antibody (34) is a mouse monoclonal IgG1 provided at 50 µg/500 µl
    • raised against amino acids 38-151 of Calretinin of rat origin
    • recommended for detection of Calretinin of mouse, rat and human origin by WB, IP and IF
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I am using Calretinin (34): sc-135853 for immunofluorescence, what dilution of this antibody should I use?

Asked by: cjMara
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from WB publication with SKOV WB publication with SKOV-3, NIH:OVCAR-3, and NIH:OVCAR-5 samples. -SCBT Publication Review
Date published: 2015-07-19
Rated 5 out of 5 by from Produced exceptional immunofluorescence cytoplasmic Produced exceptional immunofluorescence cytoplasmic staining in human endothelial cells. -SCBT QC
Date published: 2015-04-14
Rated 5 out of 5 by from Beautiful Western blot data with rat brain Beautiful Western blot data with rat brain, mouse brain and rat cerebellum tissue extracts. -SCBT QC
Date published: 2015-02-07
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