Calpain 2 Antibody (E-10)recommended for detection of Calpain 2 precursor of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Calpain 2 Antibody (E-10): sc-373966

Calpain 2 Antibody (E-10) is rated 4.8 out of 5 by 10.
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Datasheets

    • Calpain 2 Antibody (E-10) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 2-27 at the N-terminus of Calpain 2 of human origin
    • Calpain 2 Antibody (E-10) is recommended for detection of Calpain 2 precursor of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Calpain 2 Antibody (E-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-373966 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Calpain 2 (E-10): sc-373966.
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Which tissue or cell can be a positive control for calpain2 IHC ?

Asked by: hexl7
Thank you for your question. Bronchus tissue, fallopian tube, gall bladder or rectum tissues are all good positive controls for this protein for IHC.
Answered by: Tech Service
Date published: 2019-04-22

Was the antibody immunized against isoform 1 or 2 of calpain-2? Isoform 2 lacks the first 78 amino acids of isoform 1.

Asked by: Claudia_NeuroGU
Thank you for your question. Calpain 2 Antibody (E-10): sc-373966 is specific for an epitope mapping between amino acids 2-27 at the N-terminus of Calpain 2 of human origin. The immunogenic sequence is AGIAAKLAK DREAAEGLGS HDRAIKY. Since amino acids 1-78 are missing in isoform P17655-2, this isoform is not recognized by Calpain 2 Antibody (E-10): sc-373966 or Calpain 2 Antibody (F-11): sc-373967, which has been raised against the same range of amino acids.
Answered by: Technical Support Europe
Date published: 2019-01-14

What application is the blocking peptide sc-373966 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Strong Bands Clean and strong bands detected using human cardiac stem cell lysate (30ug protein). Worked at 1:500 primary dilution with 1:1000 anti-mouse secondary.
Date published: 2018-02-20
Rated 5 out of 5 by from staining image The test indicates the effectiveness of the results of image enhancement. Experiments show that it is an efficient method
Date published: 2017-10-19
Rated 5 out of 5 by from Strong signals with clean background I got the sample for western blotting using the lysates from rat cultured neurons and mouse brains, and I got strong calpain 2 signals with the clean background. I use PVDF membrane and the antibody is 1:5000 diluted in 3% milk/TBST.
Date published: 2017-09-26
Rated 5 out of 5 by from Great I got a free sample for test, and it works very well
Date published: 2017-09-07
Rated 4 out of 5 by from suitable for IF suitable for formaldehyde-fixed breast cancer cells MCF-7 by IF
Date published: 2017-08-26
Rated 5 out of 5 by from Robust and clean Antibody works pretty well and performed western blot, band is pretty clear and strong.
Date published: 2017-05-23
Rated 5 out of 5 by from strong bands Used at 1:500 to detect Calpain 2 in U87MG cells,works good,single specific band detected,staining data very strong.
Date published: 2017-02-24
Rated 5 out of 5 by from Well-rounded antibody for Calpain 2 Excellent antibody for Calpain 2, strong and specific, giving solid bands in Western blot and clean clear staining in IHC. Great choice!
Date published: 2017-01-31
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