Calpain 11 Antibody (C-2) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
raised against amino acids 1-60 mapping at the N-terminus of Calpain 11 of human origin
Calpain 11 Antibody (C-2) is recommended for detection of Calpain 11 of human origin by WB, IP, IF, IHC(P) and ELISA
Calpain 11 Antibody (C-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Calpain 11 (C-2): sc-376265.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Calpains are calcium-activated thiol proteases. They are heterodimers with one large subunit and one small subunit. The large subunit varies between family members and can be active without the small subunit. Calpains are involved in intracellular processing of proteins. Calpain 11 is a typical calpain with four domains but, in place of the typical domain IV is a similar calmodulin-like domain that associates but does not interact with the smaller regulatory subunit. Calpain 11 is found in the testis, localizing to the spermatozoa during the later stages of meiosis. It is expressed during spermatogenesis, which suggests that during meiosis and sperm functional processes, Calpain 11 may regulate calcium dependent signal transduction events.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Can Calpain 11 (C-2): sc-376265 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?
Asked by: Germaine
Thank you for your question. Yes, Calpain 11 (C-2): sc-376265 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here:
Rated 5 out of
Produced beautiful immunoperoxidase cytoplasmicProduced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human testis tissue. -SCBT QC
Date published: 2015-02-05
Rated 5 out of
Nice Western Blot data of Calpain 11 expressionNice Western Blot data of Calpain 11 expression in non-transfected and human Calpain 11 transfected 293T whole cell lysates. -SCBT QC
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