Calpain 1 Antibody (P-6)recommended for detection of Calpain 1 of human origin by WB, IP, IF and IHC(P)

Calpain 1 Antibody (P-6): sc-81171

Calpain 1 Antibody (P-6) is rated 4.0 out of 5 by 3.
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Datasheets

    • Calpain 1 Antibody (P-6) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against erythrocyte Calpain1 large subunit of human origin
    • recommended for detection of Calpain 1 of human origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See Calpain 1 (D-11): sc-271313 for Calpain 1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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I am using Calpain 1 (P-6): sc-81171 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Strong bands seen in WB using A Strong bands seen in WB using A-431, TF-1 and K-562 whole cell lysates. -SCBT QC
Date published: 2015-05-13
Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic and membrane Great immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human rectum tissue. -SCBT QC
Date published: 2015-04-16
Rated 3 out of 5 by from Robust band seen in WB using human PBL whole Robust band seen in WB using human PBL whole cell lysate. -SCBT QC
Date published: 2015-03-02
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