Calgizzarin Antibody (B-5) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
raised against amino acids 1-105 representing full length Calgizzarin of human origin
Calgizzarin Antibody (B-5) is recommended for detection of Calgizzarin of human origin by WB, IP, IF, IHC(P) and ELISA
Calgizzarin Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Calgizzarin (B-5): sc-390250.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Calgizzarin is a Ca2+-binding protein of the S100 family. The proteins comprising the S-100 protein family have two EF-hand structures per molecule of protein. Expression of these family members occurs primarily in neurons or retinal photoreceptor cells, and may also be involved in a wide variety of cellular functions including cell growth, cell-cell communication, energy metabolism and intracellular signal transduction. They have also been linked to various pathologies, including Alzheimer's disease, schizophrenia and cancer. Calgizzarin is highly expressed in lung tissue and is also expressed in kidney, liver, brain, heart and smooth muscle. It is an important androgen-responsive protein produced by Sertoli cells and might play a role in spermatogenesis regulation.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended starting dilution for Western blot for sc-390250: Calgizzarin (B-5) monoclonal antibody?
Asked by: jenni
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol:
Rated 5 out of
Ottimo funzionamentoHo comprato questo articolo meno di un mese fa, l'ho provato sia per immunofluorescenza1:100 che per western blotting 1:1000 e sono molto soddisfatta!
Date published: 2017-11-15
Rated 5 out of
Great antibody!It performed very well in my experiment. Highly recommended!
Date published: 2017-08-11
Rated 4 out of
Calgizzarin AntibodyWe used it at the concentration 1/1000 in A549 lysate. we got very clean western blot. We also tried it in tissue lysate and we got similar bands.
Date published: 2016-12-11
Rated 5 out of
Produced positive Western blot data of CalgizzarinProduced positive Western blot data of Calgizzarin expression in HeLa, PC-3, SK-BR-3 and CCRF-CEM whole cell lysates. -SCBT QC
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