Calgizzarin Antibody (B-5)recommended for detection of Calgizzarin of and human origin by WB, IP, IF, IHC(P) and ELISA

Calgizzarin Antibody (B-5): sc-390250

Calgizzarin Antibody (B-5) is rated 4.8 out of 5 by 4.
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Datasheets

    • Calgizzarin Antibody (B-5) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-105 representing full length Calgizzarin of human origin
    • Calgizzarin Antibody (B-5) is recommended for detection of Calgizzarin of human origin by WB, IP, IF, IHC(P) and ELISA
    • Calgizzarin Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Calgizzarin (B-5): sc-390250.
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What is the recommended starting dilution for Western blot for sc-390250: Calgizzarin (B-5) monoclonal antibody?

Asked by: jenni
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Ottimo funzionamento Ho comprato questo articolo meno di un mese fa, l'ho provato sia per immunofluorescenza1:100 che per western blotting 1:1000 e sono molto soddisfatta!
Date published: 2017-11-15
Rated 5 out of 5 by from Great antibody! It performed very well in my experiment. Highly recommended!
Date published: 2017-08-11
Rated 4 out of 5 by from Calgizzarin Antibody We used it at the concentration 1/1000 in A549 lysate. we got very clean western blot. We also tried it in tissue lysate and we got similar bands.
Date published: 2016-12-11
Rated 5 out of 5 by from Produced positive Western blot data of Calgizzarin Produced positive Western blot data of Calgizzarin expression in HeLa, PC-3, SK-BR-3 and CCRF-CEM whole cell lysates. -SCBT QC
Date published: 2014-02-02
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