CalciFluor™ Fluo-8, AM CAS: 1345980-40-6
MW: 1046.93

CalciFluor™ Fluo-8, AM (CAS 1345980-40-6)

CalciFluor Fluo-8, AM | CAS 1345980-40-6 is rated 5.0 out of 5 by 1.
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Alternate Names: CalciFluor™ Fluo-8, AM is known as a fluorescent probe.
Application: CalciFluor™ Fluo-8, AM is a green fluorescent calcium binding dye, a superior Ca2+ fluorescent probe than traditional Fluo-4, AM.
CAS Number: 1345980-40-6
Molecular Weight: 1046.93
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits
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CalciFluor™ Fluo-8, AM has been developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength of maximum excitation @ ~490 nm and maximum emission @ ~520 nm. Similarly to Fluo 4 AM, the anionic carboxy groups are protected with acetoxy methyl esters allowing membrane permeability, which are subsequently removed by intracellular esterases - allowing Ca2+ detection. However CalciFluor™ Fluo-8, AM is efficiently loaded into cells at room temperature (whereas Fluo-3 and Fluo-4 require much more harsh loading conditions), and CalciFluor™ Fluo-8, AM is 2 times brighter than Fluo-4 and 4 times brighter than Fluo-3. These features, as well as a lower price, make CalciFluor™ Fluo-8, AM a superior in vivo calcium indicator.


References

1. Liu L et al. TRPM7 promotes the epithelial-mesenchymal transition in ovarian cancer through the calcium-related PI3K / AKT oncogenic signaling. J Exp Clin Cancer Res 38:106 (2019).
2. Domínguez A et al. Effect of perfluorodecanoic acid on pig oocyte viability, intracellular calcium levels and gap junction intercellular communication during oocyte maturation in vitro. Toxicol In Vitro 58:224-229 (2019).
3. Bender BJ et al. Structural Model of Ghrelin Bound to its G Protein-Coupled Receptor. Structure 27:537-544.e4 (2019).
4. Datta G et al. HIV-1 gp120 Promotes Lysosomal Exocytosis in Human Schwann Cells. Front Cell Neurosci 13:329 (2019).

Physical State :
Solid
Solubility :
Soluble in DMSO, and water (low).
Storage :
Desiccate at -20° C
Fluoresence :
λex 494 nm, λem 517 nm in DMSO
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

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CalciFluor™ Fluo-8, AM (CAS 1345980-40-6)  Product Citations

See how others have used CalciFluor™ Fluo-8, AM (CAS 1345980-40-6). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 12 total

PMID: # 34033861  Özgün, A.|Garipcan, B.| et al. 2021. Biochim Biophys Acta Mol Cell Res. 1868: 119062.

PMID: # 34301262  Shen, CH.|Hsieh, CC.|Jiang, KY.|Lin, CY.|Chiang, NJ.|Li, TW.|Yen, CT.|Chen, WJ.|Hwang, DY.|Chen, LT.| et al. 2021. J Biomed Sci. 28: 55.

PMID: # 31279969  2019. Free Radic. Biol. Med. 141: 338-347.

PMID: # 28196960  von Hoven, G. et al. 2017. MBio. 8:

PMID: # 26803937  Aguettaz, E. et al. 2016. Cell Calcium. 59: 145-55.

PMID: # 27617280  Aguettaz, E. et al. 2016. Data Brief. 8: 1443-7.

PMID: # 26764221  Hussain, A. et al. 2016. Mol. Carcinog. 55: 2135-2155.

PMID: # 27190250  Wei, C. et al. 2016. Exp. Biol. Med. (Maywood). 241: 1505-15.

PMID: # 27147619  von Hoven, G. et al. 2016. Biochem. J. 473: 1929-40.

PMID: # 26470731  Lisak, D. et al. 2016. Cell Death Differ. 23: 358-68.

Citations 1 to 10 of 12 total

How long does the signal last?

Asked by: Nuruddeen
Thank you for your question. Unfortunately, we do not have this information.
Answered by: Technical Support
Date published: 2021-04-11

What is the physical appearance of this product?

Asked by: hawkeye11
Thank you for your question. This chemical, CalciFluor Fluo-8, AM, is a red solid.
Answered by: Chemical Support 1
Date published: 2017-02-23

What is the KD value of this product?

Asked by: hawkeye11
Thank you for your question. This chemical, CalciFluo Fluo-8, AM, has a Ca 2+ KD value of 389 nM.
Answered by: Chemical Support 1
Date published: 2021-04-11

What is the solubility of this product?

Asked by: hawkeye11
Thank you for your question. This chemical, CalciFluo Fluo-8, AM, is soluble in 10 mg/ml DMSO.
Answered by: Chemical Support 1
Date published: 2021-04-11
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Rated 5 out of 5 by from Various publications cite CalciFluor Fluo Various publications cite CalciFluor Fluo-8, AM as a superior Ca2+ fluorescent probe than traditional Fluo-4, AM. CalciFluor Fluo-8, AM has been developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength of maximum excitation at ~490 nm and maximum emission at ~520 nm. CalciFluor Fluo-8, AM is efficiently loaded into cells at room temperature (whereas Fluo-3 and Fluo-4 require much more harsh loading conditions), and CalciFluor Fluo-8, AM is 2 times brighter than Fluo-4 and 4 times brighter than Fluo-3. These features, as well as a lower price, make CalciFluor Fluo-8, AM a superior calcium indicator. -SCBT Publication Review
Date published: 2015-06-23
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