CalciFluor Fluo-8H, AM MW: 1074.98
A fluorescent calcium indicator with improved capability over Fluo-4 with nearly identical properties.

CalciFluor™ Fluo-8H, AM

CalciFluor Fluo-8H, AM is rated 5.0 out of 5 by 1.
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Application: CalciFluor™ Fluo-8H, AM is a fluorescent calcium indicator with improved capability over Fluo-4 with nearly identical properties
Molecular Weight: 1074.98
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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CalciFluor™ Fluo-8H, AM has been developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength of maximum excitation at ~490 nm and maximum emission at ~520 nm. Similarly to Fluo 4 AM, the anionic carboxy groups are protected with acetoxy methyl esters allowing membrane permeability, which are subsequently removed by intracellular esterases - allowing Ca2+ detection. However CalciFluor™ Fluo-8, AM is efficiently loaded into cells at room temperature (whereas Fluo-3 and Fluo-4 require much more harsh loading conditions), and CalciFluor™ Fluo-8 is 2 times brighter than Fluo-4 and 4 times brighter than Fluo-3. These features, as well as a lower price, make CalciFluor™ Fluo-8, AM a superior in vivo calcium indicator.


References

1. Baus, E., et al. 1994. J. Immunol. Methods. 173: 41-47. PMID: 8034984
2. Bailey, J.L. and Storey, B.T. 1994. Mol. Reprod. Dev. 39: 297-308. PMID: 7888169
3. Greimers, R., et al. 1996. Cytometry. 23: 205-217. PMID: 8974866
4. Bednar, B., et al. 2004. J. Neurosci. Methods. 137: 247-255. PMID: 15262068

Physical State :
Solid
Solubility :
Soluble in water (low), and DMSO.
Storage :
Desiccate at -20° C
Fluoresence :
λex 494 nm, λem 517 nm in DMSO
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

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Rated 5 out of 5 by from CalciFluor™ Fluo CalciFluor™ Fluo-8H, AM was utilized in many articles to measure calcium in vivo. -SCBT Publication Review
Date published: 2015-04-15
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