Calbindin D9K Antibody (F-6)recommended for detection of Calbindin D9K of human origin by WB, IP, IF and ELISA

Calbindin D9K Antibody (F-6): sc-74492

Calbindin D9K Antibody (F-6) is rated 5.0 out of 5 by 1.
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Datasheets

    • Calbindin D9K Antibody (F-6) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-60 mapping at the N-terminus of Calbindin D9K of human origin
    • recommended for detection of Calbindin D9K of human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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How much sample do you recommend loading on the gel for Western blot detection using Calbindin D9K (F-6): sc-74492?

Asked by: TinTin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Great Western blot data of porcine recombinant Great Western blot data of porcine recombinant Calbindin D9K. -SCBT QC
Date published: 2014-01-25
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