Calbindin D9K Antibody (D-5)recommended for detection of Calbindin D9K of human origin by WB, IP, IF, IHC(P) and ELISA

Calbindin D9K Antibody (D-5): sc-74462

Calbindin D9K Antibody (D-5) is rated 4.0 out of 5 by 5.
  • y_2019, m_12, d_5, h_17
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.3
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_en_US, sid_74462, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 4ms
  • REVIEWS, PRODUCT
5
1
4
3
3
1
2
0
1
0

Datasheets

    • Calbindin D9K Antibody (D-5) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-60 mapping at the N-terminus of Calbindin D9K of human origin
    • Calbindin D9K Antibody (D-5) is recommended for detection of Calbindin D9K of human origin by WB, IP, IF, IHC(P) and ELISA
    • Calbindin D9K Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Calbindin D9K (D-5): sc-74462.
Submit a review for this product and receive 15 CruzCredits

does D5 calbindin-d9k antibody cross react with mouse (it is indicated that the antibody detects calbindin-d9k of human origin)

Asked by: christak
Thank you for your question. We have not yet tested this antibody in mouse samples, and so it is not currently recommended for use in that species. There are a couple of product citations reporting use of this specific antibody in mouse samples. Please refer to PMID's: 21850169 and 20068228 for more details.
Answered by: Technical Support
Date published: 2019-11-06

I am using Calbindin D9K (D-5): sc-74462 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Randy McDonald
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2019, m_12, d_5, h_17CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.3
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_74462, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 3ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from works on macaque tissue Antibody detects calbindin in paraffin embedded macaque retina tissue when used at 1:50 dilution ON.
Date published: 2016-12-20
Rated 4 out of 5 by from Publishable data in CoIP using mouse hippocampal Publishable data in CoIP using mouse hippocampal Hpl 3-4 cells -SCBT Publication Review
Date published: 2015-06-19
Rated 4 out of 5 by from Publishable data in WB using mouse Retina Publishable data in WB using mouse Retina -SCBT Publication Review
Date published: 2015-03-06
Rated 5 out of 5 by from Superb immunoperoxidase cytoplasmic staining Superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human epididymis tissue. -SCBT QC
Date published: 2015-02-20
Rated 3 out of 5 by from Robust band seen in WB using pig recombinant Robust band seen in WB using pig recombinant Calbindin D9K. -SCBT QC
Date published: 2015-02-01
  • y_2019, m_12, d_5, h_17
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.3
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_en_US, sid_74462, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 3ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.