CA XII Antibody (D-2)recommended for detection of CA XII of human origin by WB, IP, IF, IHC(P) and ELISA

CA XII Antibody (D-2): sc-374314

CA XII Antibody (D-2) is rated 5.0 out of 5 by 4.
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Datasheets

    • CA XII Antibody (D-2) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 241-354 of CA XII of human origin
    • CA XII Antibody (D-2) is recommended for detection of CA XII of human origin by WB, IP, IF, IHC(P) and ELISA
    • CA XII Antibody (D-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CA XII (D-2): sc-374314.
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I am using CA XII (D-2): sc-374314 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Published WB Published WB, IP, IF and IHC(P) data with human tumor cell lines and tissues - SCBT Publication Review
Date published: 2015-05-16
Rated 5 out of 5 by from Robust signal in WB in MCF7 whole cell lysates Robust signal in WB in MCF7 whole cell lysates. -SCBT QC
Date published: 2015-02-12
Rated 5 out of 5 by from Positive signal in WB in HeLa whole cell lysate Positive signal in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-01-22
Rated 5 out of 5 by from Great immunoperoxidase membrane staining Great immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2014-02-14
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