CA VB Antibody (D-9)recommended for detection of CA VB of human origin by WB, IP, IF and ELISA

CA VB Antibody (D-9): sc-393852

CA VB Antibody (D-9) is rated 5.0 out of 5 by 3.
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Datasheets

    • CA VB Antibody (D-9) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 39-66 near the N-terminus of CA VB of human origin
    • CA VB Antibody (D-9) is recommended for detection of CA VB of human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine
    • CA VB Antibody (D-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-393852 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CA VB (D-9): sc-393852.
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What application is the blocking peptide sc-393852 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Antibody worked really well on HCT116 cells Antibody worked really well on HCT116 cells transiently transfected with pcDNA3.1 CAVB plasmid. First tried 1:100 dilution, which gave a massive band. Dilution 1:1000 worked well with a longer exposure time. I could also add an image to show the results.
Date published: 2016-07-28
Rated 5 out of 5 by from Produced great immunofluorescence cytoplasmic Produced great immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-12
Rated 5 out of 5 by from Good Western Blot data of CA VB expression Good Western Blot data of CA VB expression in 293T whole cell lysate and human liver tissue extract . -SCBT QC
Date published: 2013-02-08
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