Anti-CA IX Antibody (H-11) recommended for detection of CA IX of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-CA IX Antibody (H-11): sc-365900

Anti-CA IX Antibody (H-11) is rated 4.4 out of 5 by 9.
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Datasheets

    • Anti-CA IX Antibody (H-11) is a mouse monoclonal IgG2a κ CA IX antibody, cited in 15 publications, provided at 200 µg/ml
    • raised against amino acids 41-160 of CA IX of human origin
    • CA IX Antibody (H-11) is recommended for detection of CA IX of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-CA IX Antibody (H-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CA IX (H-11): sc-365900.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CA IX Antibody (H-11) for WB and IHC(P) applications. These reagents are now offered in bundles with CA IX Antibody (H-11) (see ordering information below).
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Hi, Is this antibody suitable for flow cytometry?

Asked by: Mark B
Thank you for your question. The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2020-05-22

What is the total volume provided of CA IX (H-11):sc-365900 monoclonal antibody?

Asked by: jenni
Thank you for your question. This antibody is provided as 200 µg in 1 ml total volume of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 7
Date published: 2017-01-13

Can CA IX (H-11): sc-365900 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, CA IX (H-11): sc-365900 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 7
Date published: 2017-01-13
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Rated 5 out of 5 by from Excellent Clean, strong band. Right molecular weight. Induced by hypoxia.
Date published: 2018-04-06
Rated 4 out of 5 by from very good I used this antibody in confocal analysis without permeabilization 1:100 dilution in DU 145 cell line
Date published: 2017-12-15
Rated 1 out of 5 by from Not detecting mouse CA9 in IF Not detecting mouse CA9 in IF of paraffin fixed tissue
Date published: 2017-12-12
Rated 5 out of 5 by from 很好用 B淋巴细胞,按说明书稀释,条带清晰,位置与预测大小一致。。。。。。。。。。。。。。。。。。。。。。。。
Date published: 2017-10-10
Rated 5 out of 5 by from Superb !! Very clear western data; also strong IHC staining!
Date published: 2017-01-20
Rated 5 out of 5 by from Good IHC and IF staining This CA IX antibody shows good results for IHC in human tissue samples.
Date published: 2017-01-12
Rated 5 out of 5 by from Positive signal in WB in human stomach tissue Positive signal in WB in human stomach tissue extract. -SCBT QC
Date published: 2015-05-15
Rated 5 out of 5 by from Robust signal in WB in HeLa whole cell lysate Robust signal in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-05-12
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